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PDBsum entry 1k5m
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Contents |
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283 a.a.
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270 a.a.
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236 a.a.
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40 a.a.
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Crystal structure of a human rhinovirus that displays part of the HIV-1 v3 loop and induces neutralizing antibodies against HIV-1.
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Authors
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J.Ding,
A.D.Smith,
S.C.Geisler,
X.Ma,
G.F.Arnold,
E.Arnold.
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Ref.
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Structure, 2002,
10,
999.
[DOI no: ]
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PubMed id
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Abstract
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We report the 2.7 A resolution structure of a chimeric rhinovirus, MN-III-2,
that displays part of the HIV-1 gp120 V3 loop and elicits HIV-neutralizing
antibodies. The V3 loop insert is dominated by two type I beta turns. The
structures of two adjacent tripeptides resemble those of analogous segments in
three Fab/V3 loop peptide complexes. Although two of the three corresponding
antibodies bind and neutralize MN-III-2 well, only one of the three can bind
without significant rearrangement. These results suggest that the V3 loop
insert: (1) can share some local conformational similarity to V3 loop sequences
presented on different structural frameworks; (2) must be able to adopt multiple
conformations, even in a relatively constrained environment; and (3) may mimic
the conformational variability of the epitope on HIV-1, increasing the
likelihood of eliciting appropriate neutralizing immune responses.
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Figure 5.
Figure 5. The Pocket Factor, Sphingosine (Shown as a Green
Ball-and-Stick Model), in the Binding Pocket of VP1 Located
below the External Canyon Region where Host Cell Receptor
Binding OccursVP1 and VP3 are shown in blue and red ribbons,
respectively. The side chains of the surrounding amino acid
residues that have close contacts with sphingosine are shown.
The interactions between sphingosine and the amino acid residues
are primarily hydrophobic in nature (=<3.6 Å, indicated with
dashed lines). Hydrogen bonds are indicated with solid lines.
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The above figure is
reprinted
by permission from Cell Press:
Structure
(2002,
10,
999-0)
copyright 2002.
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Secondary reference #1
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Title
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Human rhinovirus type 14:human immunodeficiency virus type 1 (HIV-1) v3 loop chimeras from a combinatorial library induce potent neutralizing antibody responses against HIV-1.
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Authors
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A.D.Smith,
S.C.Geisler,
A.A.Chen,
D.A.Resnick,
B.M.Roy,
P.J.Lewi,
E.Arnold,
G.F.Arnold.
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Ref.
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J Virol, 1998,
72,
651-659.
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PubMed id
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