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UniProt functional annotation for P00469 | ||
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| UniProt code: P00469. |
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| Organism: | |
Lactobacillus casei. |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lacticaseibacillus. |
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| Function: | |
Catalyzes the reductive methylation of 2'-deoxyuridine-5'- monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by- product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. {ECO:0000255|HAMAP-Rule:MF_00008}. |
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| Catalytic activity: | |
Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8- dihydrofolate + dTMP; Xref=Rhea:RHEA:12104, ChEBI:CHEBI:15636, ChEBI:CHEBI:57451, ChEBI:CHEBI:63528, ChEBI:CHEBI:246422; EC=2.1.1.45; Evidence={ECO:0000255|HAMAP-Rule:MF_00008}; |
| Pathway: | |
Pyrimidine metabolism; dTTP biosynthesis. {ECO:0000255|HAMAP- Rule:MF_00008}. |
| Subunit: | |
Homodimer. {ECO:0000255|HAMAP-Rule:MF_00008}. |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00008}. |
| Similarity: | |
Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily. {ECO:0000255|HAMAP-Rule:MF_00008}. |
| Sequence caution: | |
Sequence=AAA25255.1; Type=Erroneous initiation; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.