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PDBsum entry 1jgo
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Contents |
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234 a.a.*
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206 a.a.*
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208 a.a.*
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150 a.a.*
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101 a.a.*
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155 a.a.*
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138 a.a.*
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127 a.a.*
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98 a.a.*
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119 a.a.*
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124 a.a.*
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125 a.a.*
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60 a.a.*
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88 a.a.*
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83 a.a.*
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104 a.a.*
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73 a.a.*
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80 a.a.*
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99 a.a.*
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24 a.a.*
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* C-alpha coords only
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References listed in PDB file
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Key reference
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Title
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The path of messenger RNA through the ribosome.
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Authors
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G.Z.Yusupova,
M.M.Yusupov,
J.H.Cate,
H.F.Noller.
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Ref.
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Cell, 2001,
106,
233-241.
[DOI no: ]
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PubMed id
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Abstract
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Using X-ray crystallography, we have directly observed the path of mRNA in the
70S ribosome in Fourier difference maps at 7 A resolution. About 30 nucleotides
of the mRNA are wrapped in a groove that encircles the neck of the 30S subunit.
The Shine-Dalgarno helix is bound in a large cleft between the head and the back
of the platform. At the interface, only about eight nucleotides (-1 to +7),
centered on the junction between the A and P codons, are exposed, and bond
almost exclusively to 16S rRNA. The mRNA enters the ribosome around position +13
to +15, the location of downstream pseudoknots that stimulate -1 translational
frame shifting.
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Figure 1.
Figure 1. Nucleotide Sequences of the Three Model mRNAs
Used in this StudyThe Shine-Dalgarno sequence (S/D), and P- and
A-site codons are underlined. The self-complementary sequences
forming the putative A-site helix in MF36 mRNA are overlined
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Figure 2.
Figure 2. Fourier Difference Maps of mRNAs(A) 7 Å
Fourier difference map of MK27 mRNA with the mRNA model (yellow)
docked, showing the position of the Shine-Dalgarno (S/D) helix
(magenta) and the positions of the A- and P-site codons (orange
and red, respectively), viewed from the top of the 30S
ribosomal subunit.(B) Difference map of the MF36 mRNA, showing a
four-base-pair tetraloop helix (A-site helix) fitted to the
extra density at the A site.(C) Same as for (B), except that the
A-tRNA anticodon stem-loop (green) is shown in the position
observed experimentally in the A-tRNA difference map (Yusupov et
al., 2001), in place of the A-site mRNA helix. The
five-nucleotide (GGAGG/CCUCC) core of the Shine-Dalgarno
interaction is shown in magenta, and the rest of the 16S rRNA
tail in cyan
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The above figures are
reprinted
by permission from Cell Press:
Cell
(2001,
106,
233-241)
copyright 2001.
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Headers
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