PDBsum entry 1jev

Complex (peptide transport/peptide)

PDB id: 1jev

Contents

Protein chain

517 a.a. *

Ligands

LYS-TRP-LYS

Metals

IUM ×8

Waters ×522

* Residue conservation analysis

References listed in PDB file

Key reference

Title

The role of water in sequence-Independent ligand binding by an oligopeptide transporter protein.

Authors

J.R.Tame, S.H.Sleigh, A.J.Wilkinson, J.E.Ladbury.

Ref.

Nat Struct Biol, 1996, 3, 998.

PubMed id

8946852

Abstract

No abstract given.

Secondary reference #1

Title

The crystal structures of the oligopeptide-Binding protein oppa complexed with tripeptide and tetrapeptide ligands.

Authors

J.R.Tame, E.J.Dodson, G.Murshudov, C.F.Higgins, A.J.Wilkinson.

Ref.

Structure, 1995, 3, 1395-1406. [DOI no: 10.1016/S0969-2126(01)00276-3]

PubMed id

8747465

Figure 1.
Figure 1. Stereo Cα trace of OppA in the closed, ligand bound form. The tri-lysine ligand is shown in thicker lines. Figure 1. Stereo Cα trace of OppA in the closed, ligand bound form. The tri-lysine ligand is shown in thicker lines.

Figure 5.
Figure 5. . Schematic diagram illustrating the interactions made by the main chain of the tri-lysine ligand with OppA. Protein residues are labelled. Hydrogen bonding and electrostatic interactions are indicated by the dotted lines. R1, R2 and R3 indicate the ligand side chains. Figure 5. . Schematic diagram illustrating the interactions made by the main chain of the tri-lysine ligand with OppA. Protein residues are labelled. Hydrogen bonding and electrostatic interactions are indicated by the dotted lines. R1, R2 and R3 indicate the ligand side chains.

The above figures are reproduced from the cited reference with permission from Cell Press

Secondary reference #2

Title

Structure determination of oppa at 2.3 a resolution using multiple-Wavelength anomalous dispersion methods.

Authors

I.D.Glover, R.C.Denny, N.D.Nguti, S.M.Mcsweeney, S.H.Kinder, A.W.Thompson, E.J.Dodson, A.J.Wilkinson, J.R.Tame.

Ref.

Acta Crystallogr D Biol Crystallogr, 1995, 51, 39-47. [DOI no: 10.1107/S090744499400692X]

PubMed id

15299334

Figure 2.
Fig. 2. Harker section, (w = 1/2), of the Patterson maps calculated with coefficients (a) anomalous differences recorded at a wavelength of 0.719 , corresponding to the white­line feature observed in XANES spectrum of OppA­L and (b) dispersive differences between data sets ,A~ and ,12. The Harker vectors corresponding to self vectors (+) and cross vectors ( ) resulting from the Patterson search procedue of SHELX90 are superimposed.

The above figure is reproduced from the cited reference with permission from the IUCr

Secondary reference #3

Title

The structural basis of sequence-Independent peptide binding by oppa protein.

Authors

J.R.Tame, G.N.Murshudov, E.J.Dodson, T.K.Neil, G.G.Dodson, C.F.Higgins, A.J.Wilkinson.

Ref.

Science, 1994, 264, 1578-1581. [DOI no: 10.1126/science.8202710]

PubMed id

8202710