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PDBsum entry 1jeh
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Oxidoreductase
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PDB id
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1jeh
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Crystal structure of eucaryotic e3, Lipoamide dehydrogenase from yeast.
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Authors
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T.Toyoda,
K.Suzuki,
T.Sekiguchi,
L.J.Reed,
A.Takenaka.
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Ref.
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J Biochem (tokyo), 1998,
123,
668-674.
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PubMed id
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Abstract
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The crystal structure of eucaryotic lipoamide dehydrogenase from yeast has been
determined by an X-ray analysis at 2.7 (partially at 2.4) A resolution. The
enzyme has two identical subunits related by a pseudo twofold symmetry. The
tertiary structure is similar to those of other procaryotic enzymes. The active
site, consisting of FAD, Cys44, and Cys49 from one subunit and His457' from the
other subunit, is highly conserved. This enzyme is directly bound to the core
protein E2 of the 2-oxoglutarate dehydrogenase complex, whereas it is bound to
the pyruvate dehydrogenase complex through a protein X. The calculated
electrostatic potential suggests two characteristic regions for binding with
these two proteins.
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