UniProt functional annotation for Q9UJY5



	UniProt functional annotation for Q9UJY5

UniProt code: Q9UJY5.

Organism: Homo sapiens (Human).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Function: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif (PubMed:11301005, PubMed:15886016). Mediates export of the GPCR receptor ADRA2B to the cell surface (PubMed:27901063). Required for targeting PKD1:PKD2 complex from the trans-Golgi network to the cilium membrane (By similarity). Regulates retrograde transport of proteins such as phosphorylated form of BACE1 from endosomes to the trans-Golgi network (PubMed:15886016, PubMed:15615712). {ECO:0000250|UniProtKB:Q8R0H9, ECO:0000269|PubMed:11301005, ECO:0000269|PubMed:15615712, ECO:0000269|PubMed:15886016, ECO:0000269|PubMed:27901063}.

Subunit: Monomer (Probable). Interacts with GGA2 and GGA3 (PubMed:14638859). Binds to clathrin and activated ARFs, including ARF1, ARF5 and ARF6 (PubMed:11301005, PubMed:11950392, PubMed:12679809, PubMed:15143060, PubMed:22522702). Interacts with RABEP1 (PubMed:12505986, PubMed:15143060, PubMed:14665628). Interacts with RABGEF1 (PubMed:12505986, PubMed:15143060). Interacts with the type-I membrane proteins LRP3, M6PR/CD-MPR and IGF2R/CI-MPR (PubMed:11390366, PubMed:11387475, PubMed:12060753). Interacts (via N-terminal VHS domain) with SORL1/sorLA and SORT1 (via C-terminal cytosolic domain) (PubMed:11821067, PubMed:17855360, PubMed:20015111). Interacts with EPN4 (PubMed:12538641). Interacts with CCDC91 (PubMed:12808037, PubMed:12858163). Interacts with HEATR5B/p200a (PubMed:12808037, PubMed:15758025). Interacts with SYNRG/gamma-synergin (PubMed:10814529, PubMed:12808037, PubMed:15758025). Interacts (via GAE doamin) with NECAP1 and NECAP2 (PubMed:14665628). Interacts (via GAE domain) with AFTPH/aftiphilin (PubMed:14665628, PubMed:15758025). Interacts with TSG101 and UBC (PubMed:14660606, PubMed:15143060). Interacts with RNF11 (PubMed:20676133). Interacts (via VHS domain) with BACE1 (via DXXLL motif); the interaction highly increases when BACE1 is phosphorylated at 'Ser-498' (PubMed:14567678, PubMed:15886016). Interacts with CNST (By similarity). Interacts with ADRA2B (PubMed:27901063). Interacts with ARL3; the interaction recruits, in collaboration with RABEP1, PKD1:PKD2 complex to trans-Golgi network and is required for ciliary targeting (By similarity). {ECO:0000250|UniProtKB:Q8R0H9, ECO:0000269|PubMed:10814529, ECO:0000269|PubMed:11301005, ECO:0000269|PubMed:11387475, ECO:0000269|PubMed:11390366, ECO:0000269|PubMed:11821067, ECO:0000269|PubMed:11950392, ECO:0000269|PubMed:12060753, ECO:0000269|PubMed:12505986, ECO:0000269|PubMed:12538641, ECO:0000269|PubMed:12679809, ECO:0000269|PubMed:12808037, ECO:0000269|PubMed:12858163, ECO:0000269|PubMed:14567678, ECO:0000269|PubMed:14638859, ECO:0000269|PubMed:14660606, ECO:0000269|PubMed:14665628, ECO:0000269|PubMed:15143060, ECO:0000269|PubMed:15758025, ECO:0000269|PubMed:15886016, ECO:0000269|PubMed:17855360, ECO:0000269|PubMed:20015111, ECO:0000269|PubMed:20676133, ECO:0000269|PubMed:22522702, ECO:0000269|PubMed:27901063, ECO:0000305}.

Subcellular location: Golgi apparatus, trans-Golgi network membrane {ECO:0000269|PubMed:15886016}; Peripheral membrane protein. Endosome membrane {ECO:0000269|PubMed:15886016}; Peripheral membrane protein. Early endosome membrane {ECO:0000269|PubMed:15886016}; Peripheral membrane protein {ECO:0000305}.

Tissue specificity: Ubiquitously expressed.

Domain: The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (DXXLL motif).

Domain: The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis. {ECO:0000269|PubMed:15143060}.

Domain: The unstructured hinge region contains clathrin-binding but no autoinhibitory (DXXLL) motifs. {ECO:0000269|PubMed:11301005}.

Domain: The GAE domain binds accessory proteins regulating GGAs function.

Ptm: Phosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal DXXLL motif to bind the VHS domain and to inhibit the recognition of cargo signals. {ECO:0000269|PubMed:12060753}.

Ptm: Ubiquitinated. {ECO:0000250}.

Similarity: Belongs to the GGA protein family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Homo sapiens (Human).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.



Function:		Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif (PubMed:11301005, PubMed:15886016). Mediates export of the GPCR receptor ADRA2B to the cell surface (PubMed:27901063). Required for targeting PKD1:PKD2 complex from the trans-Golgi network to the cilium membrane (By similarity). Regulates retrograde transport of proteins such as phosphorylated form of BACE1 from endosomes to the trans-Golgi network (PubMed:15886016, PubMed:15615712). {ECO:0000250\|UniProtKB:Q8R0H9, ECO:0000269\|PubMed:11301005, ECO:0000269\|PubMed:15615712, ECO:0000269\|PubMed:15886016, ECO:0000269\|PubMed:27901063}.


Subunit:		Monomer (Probable). Interacts with GGA2 and GGA3 (PubMed:14638859). Binds to clathrin and activated ARFs, including ARF1, ARF5 and ARF6 (PubMed:11301005, PubMed:11950392, PubMed:12679809, PubMed:15143060, PubMed:22522702). Interacts with RABEP1 (PubMed:12505986, PubMed:15143060, PubMed:14665628). Interacts with RABGEF1 (PubMed:12505986, PubMed:15143060). Interacts with the type-I membrane proteins LRP3, M6PR/CD-MPR and IGF2R/CI-MPR (PubMed:11390366, PubMed:11387475, PubMed:12060753). Interacts (via N-terminal VHS domain) with SORL1/sorLA and SORT1 (via C-terminal cytosolic domain) (PubMed:11821067, PubMed:17855360, PubMed:20015111). Interacts with EPN4 (PubMed:12538641). Interacts with CCDC91 (PubMed:12808037, PubMed:12858163). Interacts with HEATR5B/p200a (PubMed:12808037, PubMed:15758025). Interacts with SYNRG/gamma-synergin (PubMed:10814529, PubMed:12808037, PubMed:15758025). Interacts (via GAE doamin) with NECAP1 and NECAP2 (PubMed:14665628). Interacts (via GAE domain) with AFTPH/aftiphilin (PubMed:14665628, PubMed:15758025). Interacts with TSG101 and UBC (PubMed:14660606, PubMed:15143060). Interacts with RNF11 (PubMed:20676133). Interacts (via VHS domain) with BACE1 (via DXXLL motif); the interaction highly increases when BACE1 is phosphorylated at 'Ser-498' (PubMed:14567678, PubMed:15886016). Interacts with CNST (By similarity). Interacts with ADRA2B (PubMed:27901063). Interacts with ARL3; the interaction recruits, in collaboration with RABEP1, PKD1:PKD2 complex to trans-Golgi network and is required for ciliary targeting (By similarity). {ECO:0000250\|UniProtKB:Q8R0H9, ECO:0000269\|PubMed:10814529, ECO:0000269\|PubMed:11301005, ECO:0000269\|PubMed:11387475, ECO:0000269\|PubMed:11390366, ECO:0000269\|PubMed:11821067, ECO:0000269\|PubMed:11950392, ECO:0000269\|PubMed:12060753, ECO:0000269\|PubMed:12505986, ECO:0000269\|PubMed:12538641, ECO:0000269\|PubMed:12679809, ECO:0000269\|PubMed:12808037, ECO:0000269\|PubMed:12858163, ECO:0000269\|PubMed:14567678, ECO:0000269\|PubMed:14638859, ECO:0000269\|PubMed:14660606, ECO:0000269\|PubMed:14665628, ECO:0000269\|PubMed:15143060, ECO:0000269\|PubMed:15758025, ECO:0000269\|PubMed:15886016, ECO:0000269\|PubMed:17855360, ECO:0000269\|PubMed:20015111, ECO:0000269\|PubMed:20676133, ECO:0000269\|PubMed:22522702, ECO:0000269\|PubMed:27901063, ECO:0000305}.
Subcellular location:		Golgi apparatus, trans-Golgi network membrane {ECO:0000269\|PubMed:15886016}; Peripheral membrane protein. Endosome membrane {ECO:0000269\|PubMed:15886016}; Peripheral membrane protein. Early endosome membrane {ECO:0000269\|PubMed:15886016}; Peripheral membrane protein {ECO:0000305}.
Tissue specificity:		Ubiquitously expressed.
Domain:		The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (DXXLL motif).
Domain:		The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis. {ECO:0000269\|PubMed:15143060}.
Domain:		The unstructured hinge region contains clathrin-binding but no autoinhibitory (DXXLL) motifs. {ECO:0000269\|PubMed:11301005}.
Domain:		The GAE domain binds accessory proteins regulating GGAs function.
Ptm:		Phosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal DXXLL motif to bind the VHS domain and to inhibit the recognition of cargo signals. {ECO:0000269\|PubMed:12060753}.
Ptm:		Ubiquitinated. {ECO:0000250}.
Similarity:		Belongs to the GGA protein family. {ECO:0000305}.