|
|
||
|
|
|
|
|
UniProt functional annotation for O73947 | ||
|
|
|
|
|
|
||
| UniProt code: O73947. |
|
||
| Organism: | |
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1). |
| Taxonomy: | |
Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; Pyrococcus. |
|
||
|
||
| Function: | |
Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication. Unlike its eukaryotic paralog, loads on circular DNA without the replication factor C (RFC) clamp loader, although RFC greatly increases loading efficiency. Stimulates the ATPase activity of replication factor C (RFC) in the presence of ssDNA. Stimulates the helicase activity of Hel308 and may alter its substrate specificity. {ECO:0000269|PubMed:11274122, ECO:0000269|PubMed:12296822, ECO:0000269|PubMed:16436047}. |
|
||
| Subunit: | |
Homotrimer which circularizes head-to-tail (head is a N- terminus, tail is at C-terminus) to form a toroid. RFC opens the toroid so it can load on DNA. Interacts with both Pol I (pol) and Pol II (polB-polC), with Hel308 (hjm) and with Hjc. Interaction with the C- terminal PIP-box of RfcL may stabilize the toroidal structure. {ECO:0000269|PubMed:10430863, ECO:0000269|PubMed:11266590, ECO:0000269|PubMed:12296822, ECO:0000269|PubMed:12649440, ECO:0000269|PubMed:16436047}. |
| Similarity: | |
Belongs to the PCNA family. {ECO:0000255|HAMAP- Rule:MF_00317}. |
Annotations taken from UniProtKB at the EBI.