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PDBsum entry 1io8
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Oxidoreductase
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PDB id
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1io8
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Thermophilic cytochrome p450 (cyp119) from sulfolobus solfataricus: high resolution structure and functional properties.
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Authors
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S.Y.Park,
K.Yamane,
S.Adachi,
Y.Shiro,
K.E.Weiss,
S.A.Maves,
S.G.Sligar.
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Ref.
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J Inorg Biochem, 2002,
91,
491-501.
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PubMed id
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Abstract
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Crystal structures of a thermostable cytochrome P450 (CYP119) and a
site-directed mutant, (Phe24Leu), from the acidothermophilic archaea Sulfolobus
solfataricus were determined at 1.5-2.0 A resolution. We identify important
crystallographic waters in the ferric heme pocket, observe protein
conformational changes upon inhibitor binding, and detect a unique distribution
of surface charge not found in other P450s. An analysis of factors contributing
to thermostability of CYP119 of these high resolution structures shows an
apparent increase in clustering of aromatic residues and optimum stacking. The
contribution of aromatic stacking was investigated further with the mutant
crystal structure and differential scanning calorimetry.
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