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UniProt functional annotation for P38489 | ||
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| UniProt code: P38489. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4- dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species. {ECO:0000269|PubMed:15684426}. |
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| Catalytic activity: | |
Reaction=5,6,7,8-tetrahydropteridine + NAD(+) = 6,7-dihydropteridine + H(+) + NADH; Xref=Rhea:RHEA:17869, ChEBI:CHEBI:15378, ChEBI:CHEBI:28889, ChEBI:CHEBI:30156, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.5.1.34; |
| Catalytic activity: | |
Reaction=5,6,7,8-tetrahydropteridine + NADP(+) = 6,7-dihydropteridine + H(+) + NADPH; Xref=Rhea:RHEA:17865, ChEBI:CHEBI:15378, ChEBI:CHEBI:28889, ChEBI:CHEBI:30156, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.5.1.34; |
| Cofactor: | |
Name=FMN; Xref=ChEBI:CHEBI:58210; |
| Activity regulation: | |
Subject to competitive inhibition by dicoumarol, nicotinic acid and acetate with respect to NADH. Subject to uncompetitive inhibition by dicoumarol, nicotinic acid and acetate with respect to nitrofurazone and nitrofurantoin. {ECO:0000269|PubMed:15684426}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=350 uM for NADH {ECO:0000269|PubMed:15684426}; KM=1850 uM for nitrofurazone {ECO:0000269|PubMed:15684426}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:11020276, ECO:0000269|PubMed:11491290, ECO:0000269|PubMed:12954054, ECO:0000269|PubMed:15684426}. |
| Similarity: | |
Belongs to the nitroreductase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.