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PDBsum entry 1hlh
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DNA-binding regulatory protein
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PDB id
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1hlh
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References listed in PDB file
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Key reference
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Title
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Proposed structure for the DNA-Binding domain of the helix-Loop-Helix family of eukaryotic gene regulatory proteins.
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Authors
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T.J.Gibson,
J.D.Thompson,
R.A.Abagyan.
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Ref.
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Protein Eng, 1993,
6,
41-50.
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PubMed id
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Abstract
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A modelled tertiary structure for the dimeric HLH domain of the E47 protein is
presented. Structural information was obtained from the aligned sequences of
> 40 members of the HLH family. The information was used to model each
monomer as an alpha-helical hairpin, with knobs-into-holes packing of
side-chains as found in antiparallel coiled-coil. The dimer forms a four-helix
bundle with additional knobs-into-holes packing at the dimer interface. The size
and electrostatic properties of core-forming residues are all accounted for in
the model. The model does not violate any known properties of protein structure.
The monomers are related by two-fold rotational symmetry, in agreement with the
observed DNA-binding sites which are imperfect inverted repeats. The N-terminal
basic region, in which DNA binding and base specificity reside, forms the first
part of helix 1. A prediction based on the model structure is that the HLH
domains do not bind to DNA in its B form but require a partially unwound
conformation in order to enter the major groove.
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Secondary reference #1
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Title
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Rop/helix-Loop-Helix similarity.
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Authors
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T.J.Gibson,
P.R.Sibbald,
P.Rice.
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Ref.
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Dna Seq, 1991,
1,
213-215.
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PubMed id
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