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UniProt functional annotation for P30043 | ||
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| UniProt code: P30043. |
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| Organism: | |
Homo sapiens (Human). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. |
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| Function: | |
Broad specificity oxidoreductase that catalyzes the NADPH- dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to heme catabolism and metabolizes linear tetrapyrroles. Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. In the liver, converts biliverdin to bilirubin. {ECO:0000269|PubMed:10620517}. |
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| Catalytic activity: | |
Reaction=NADP(+) + reduced riboflavin = 2 H(+) + NADPH + riboflavin; Xref=Rhea:RHEA:19377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17607, ChEBI:CHEBI:57783, ChEBI:CHEBI:57986, ChEBI:CHEBI:58349; EC=1.5.1.30; Evidence={ECO:0000269|PubMed:10620517, ECO:0000269|PubMed:18241201}; |
| Catalytic activity: | |
Reaction=bilirubin IXalpha + NAD(+) = biliverdin IXalpha + H(+) + NADH; Xref=Rhea:RHEA:15797, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:57977, ChEBI:CHEBI:57991; EC=1.3.1.24; Evidence={ECO:0000269|PubMed:10620517, ECO:0000269|PubMed:18241201, ECO:0000269|PubMed:7929092}; |
| Catalytic activity: | |
Reaction=bilirubin IXalpha + NADP(+) = biliverdin IXalpha + H(+) + NADPH; Xref=Rhea:RHEA:15793, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:57977, ChEBI:CHEBI:57991, ChEBI:CHEBI:58349; EC=1.3.1.24; Evidence={ECO:0000269|PubMed:10620517, ECO:0000269|PubMed:18241201, ECO:0000269|PubMed:7929092}; |
| Activity regulation: | |
Mesobiliverdin acts as competitve inhibitor for flavin reduction, indicating that flavin and tetrapyrrole substrates compete for the same site. {ECO:0000269|PubMed:10620517}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=36 uM for NADP {ECO:0000269|PubMed:10620517, ECO:0000269|PubMed:7929092}; KM=5.6 mM for NADH {ECO:0000269|PubMed:10620517, ECO:0000269|PubMed:7929092}; KM=0.3 uM for biliverdin IX-beta {ECO:0000269|PubMed:10620517, ECO:0000269|PubMed:7929092}; KM=52 uM for FMN {ECO:0000269|PubMed:10620517, ECO:0000269|PubMed:7929092}; KM=125 uM for FAD {ECO:0000269|PubMed:10620517, ECO:0000269|PubMed:7929092}; KM=53 uM for riboflavin {ECO:0000269|PubMed:10620517, ECO:0000269|PubMed:7929092}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:11224564, ECO:0000269|PubMed:7929092}. |
| Subcellular location: | |
Cytoplasm {ECO:0000269|PubMed:7929092}. |
| Tissue specificity: | |
Predominantly expressed in liver and erythrocytes. At lower levels in heart, lung, adrenal gland and cerebrum. {ECO:0000269|PubMed:7929092}. |
Annotations taken from UniProtKB at the EBI.