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UniProt functional annotation for P0A953 | ||
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| UniProt codes: P0A953, P14926. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Involved in the type II fatty acid elongation cycle. Catalyzes the elongation of a wide range of acyl-ACP by the addition of two carbons from malonyl-ACP to an acyl acceptor (PubMed:9013860, PubMed:8910376, PubMed:19679654, PubMed:22017312). Can also use unsaturated fatty acids (PubMed:3076377, PubMed:8910376, PubMed:19679654). Catalyzes a key reaction in unsaturated fatty acid (UFA) synthesis, the elongation of the cis-3-decenoyl-ACP produced by FabA (PubMed:19679654). Can use acyl chains from C-6 to C-14 (PubMed:9013860, PubMed:8910376, PubMed:19679654, PubMed:22017312). Has an absolute requirement for an ACP substrate as the acyl donor, and no activity is detected when both substrates are based on CoA (PubMed:22017312). {ECO:0000269|PubMed:19679654, ECO:0000269|PubMed:22017312, ECO:0000269|PubMed:3076377, ECO:0000269|PubMed:8910376, ECO:0000269|PubMed:9013860}. |
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| Catalytic activity: | |
Reaction=a fatty acyl-[ACP] + H(+) + malonyl-[ACP] = a 3-oxoacyl-[ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:22836, Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9685, Rhea:RHEA-COMP:9916, Rhea:RHEA-COMP:14125, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, ChEBI:CHEBI:78449, ChEBI:CHEBI:78776, ChEBI:CHEBI:138651; EC=2.3.1.41; Evidence={ECO:0000269|PubMed:19679654, ECO:0000269|PubMed:22017312, ECO:0000269|PubMed:8910376, ECO:0000269|PubMed:9013860}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22837; Evidence={ECO:0000269|PubMed:19679654, ECO:0000269|PubMed:22017312, ECO:0000269|PubMed:8910376, ECO:0000269|PubMed:9013860}; |
| Catalytic activity: | |
Reaction=(3Z)-decenoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxo-(5Z)- dodecenoyl-[ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:54940, Rhea:RHEA- COMP:9623, Rhea:RHEA-COMP:9685, Rhea:RHEA-COMP:9927, Rhea:RHEA- COMP:14042, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, ChEBI:CHEBI:78449, ChEBI:CHEBI:78798, ChEBI:CHEBI:138410; Evidence={ECO:0000269|PubMed:19679654}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:54941; Evidence={ECO:0000269|PubMed:19679654}; |
| Catalytic activity: | |
Reaction=H(+) + hexanoyl-[ACP] + malonyl-[ACP] = 3-oxooctanoyl-[ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41836, Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9632, Rhea:RHEA-COMP:9633, Rhea:RHEA-COMP:9685, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, ChEBI:CHEBI:78449, ChEBI:CHEBI:78459, ChEBI:CHEBI:78460; Evidence={ECO:0000269|PubMed:9013860}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41837; Evidence={ECO:0000269|PubMed:9013860}; |
| Catalytic activity: | |
Reaction=H(+) + malonyl-[ACP] + octanoyl-[ACP] = 3-oxodecanoyl-[ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41852, Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9636, Rhea:RHEA-COMP:9637, Rhea:RHEA-COMP:9685, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, ChEBI:CHEBI:78449, ChEBI:CHEBI:78463, ChEBI:CHEBI:78464; Evidence={ECO:0000269|PubMed:9013860}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41853; Evidence={ECO:0000269|PubMed:9013860}; |
| Catalytic activity: | |
Reaction=decanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxododecanoyl-[ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41868, Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9640, Rhea:RHEA-COMP:9641, Rhea:RHEA-COMP:9685, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, ChEBI:CHEBI:78449, ChEBI:CHEBI:78468, ChEBI:CHEBI:78469; Evidence={ECO:0000269|PubMed:9013860}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41869; Evidence={ECO:0000269|PubMed:9013860}; |
| Catalytic activity: | |
Reaction=dodecanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxotetradecanoyl- [ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41884, Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9644, Rhea:RHEA-COMP:9645, Rhea:RHEA-COMP:9685, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, ChEBI:CHEBI:65264, ChEBI:CHEBI:78449, ChEBI:CHEBI:78473; Evidence={ECO:0000269|PubMed:22017312, ECO:0000269|PubMed:9013860}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41885; Evidence={ECO:0000269|PubMed:22017312, ECO:0000269|PubMed:9013860}; |
| Catalytic activity: | |
Reaction=H(+) + malonyl-[ACP] + tetradecanoyl-[ACP] = 3- oxohexadecanoyl-[ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41900, Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9648, Rhea:RHEA-COMP:9649, Rhea:RHEA-COMP:9685, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, ChEBI:CHEBI:78449, ChEBI:CHEBI:78477, ChEBI:CHEBI:78478; Evidence={ECO:0000269|PubMed:8910376}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41901; Evidence={ECO:0000269|PubMed:8910376}; |
| Catalytic activity: | |
Reaction=(7Z)-tetradecenoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxo-(9Z)- hexadecenoyl-[ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:54924, Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9685, Rhea:RHEA-COMP:14038, Rhea:RHEA-COMP:14039, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, ChEBI:CHEBI:78449, ChEBI:CHEBI:138405, ChEBI:CHEBI:138406; Evidence={ECO:0000269|PubMed:8910376}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:54925; Evidence={ECO:0000269|PubMed:8910376}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=11.5 uM for malonyl-[ACP] (in the presence of dodecanoyl-[ACP]) {ECO:0000269|PubMed:22017312}; KM=3.2 uM for dodecanoyl-[ACP] (in the presence of malonyl-[ACP]) {ECO:0000269|PubMed:22017312}; KM=153 uM for malonyl-CoA (in the presence of dodecanoyl-[ACP]) {ECO:0000269|PubMed:22017312}; KM=58.6 uM for dodecanoyl-CoA (in the presence of malonyl-[ACP]) {ECO:0000269|PubMed:22017312}; Note=kcat is 6.6 min(-1) with malonyl-[ACP] as substrate in the presence of dodecanoyl-[ACP]. kcat is 3.4 min(-1) with dodecanoyl- [ACP] in the presence of malonyl-[ACP]. {ECO:0000269|PubMed:22017312}; |
| Pathway: | |
Lipid metabolism; fatty acid biosynthesis. {ECO:0000305|PubMed:19679654, ECO:0000305|PubMed:8910376}. |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:10571059, ECO:0000269|PubMed:9013860}. |
| Subcellular location: | |
Cytoplasm. |
| Induction: | |
Mainly activated by FadR, but minor repression is also conferred by FabR (PubMed:11859088, PubMed:21276098). {ECO:0000269|PubMed:11859088, ECO:0000269|PubMed:21276098}. |
| Similarity: | |
Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.