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PDBsum entry 1gxq
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Transcriptional activator
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PDB id
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1gxq
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Tandem DNA recognition by phob, A two-Component signal transduction transcriptional activator.
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Authors
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A.G.Blanco,
M.Sola,
F.X.Gomis-Rüth,
M.Coll.
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Ref.
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Structure, 2002,
10,
701-713.
[DOI no: ]
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PubMed id
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Abstract
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PhoB is a signal transduction response regulator that activates nearly 40 genes
in phosphate depletion conditions in E. coli and closely related bacteria. The
structure of the PhoB effector domain in complex with its target DNA sequence,
or pho box, reveals a novel tandem arrangement in which several monomers bind
head to tail to successive 11-base pair direct-repeat sequences, coating one
face of a smoothly bent double helix. The protein has a winged helix fold in
which the DNA recognition elements comprise helix alpha 3, penetrating the major
groove, and a beta hairpin wing interacting with a compressed minor groove via
Arg219, tightly sandwiched between the DNA sugar backbones. The transactivation
loops protrude laterally in an appropriate orientation to interact with the RNA
polymerase sigma(70) subunit, which triggers transcription initiation.
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Figure 6.
Figure 6. Proposed Mechanism of InhibitionSuperposition of
two full-length DrrD molecules (pink and green) with two PhoB^RD
and two PhoB^DBTD bound to DNA (colored as in Figure 2B) showing
steric clashes between adjacent monomers.
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The above figure is
reprinted
by permission from Cell Press:
Structure
(2002,
10,
701-713)
copyright 2002.
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