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PDBsum entry 1gnc
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Growth factor
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PDB id
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1gnc
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References listed in PDB file
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Key reference
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Title
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Structure and dynamics of the human granulocyte colony-Stimulating factor determined by nmr spectroscopy. Loop mobility in a four-Helix-Bundle protein.
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Authors
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T.Zink,
A.Ross,
K.Lüers,
C.Cieslar,
R.Rudolph,
T.A.Holak.
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Ref.
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Biochemistry, 1994,
33,
8453-8463.
[DOI no: ]
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PubMed id
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Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
perfect match.
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Abstract
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Recombinant 15N- and 13C-labeled human granulocyte colony-stimulating factor
(rh-metG-CSF) has been studied by 2D and 3D NMR using uniformly labeled protein,
as well as residue-specific 15N-labeled samples. Assignment of 90% of the
backbone resonances and 85% of side-chain resonances has enabled the
determination of both the secondary and tertiary structures of the protein. The
fold is similar to those of the human growth hormone and other growth factors.
Four stretches of helices were identified between residues 11 and 41 (helix A),
71 and 95 (helix B), 102 and 125 (helix C), and 145 and 170 (helix D), which
form a left-handed four-helix bundle with helices A and B aligned parallel to
one another (up-up) and antiparallel to helices C and D (down-down). An
additional short fifth helix (E) is part of the AB loop connecting helices A and
B. Examination of the protein's relaxation behavior, based on the model-free
approach of Lipari and Szabo, shows that the G-CSF backbone has a well-defined
structure of limited conformational flexibility in helices. In contrast, the
long loop connecting helices C and D exhibits substantial fast internal motion
compared to the overall rotational correlation time of the whole molecule, which
is on the order of 13 ns.
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Secondary reference #1
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Title
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Secondary structure of human granulocyte colony-Stimulating factor derived from nmr spectroscopy.
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Authors
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T.Zink,
A.Ross,
D.Ambrosius,
R.Rudolph,
T.A.Holak.
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Ref.
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FEBS Lett, 1992,
314,
435-439.
[DOI no: ]
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PubMed id
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