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UniProt functional annotation for P08536 | ||
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| UniProt code: P08536. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
Catalyzes the first intracellular reaction of sulfate assimilation, forming adenosine-5'-phosphosulfate (APS) from inorganic sulfate and ATP. Plays an important role in sulfate activation as a component of the biosynthesis pathway of sulfur-containing amino acids. {ECO:0000255|HAMAP-Rule:MF_03106}. |
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| Catalytic activity: | |
Reaction=ATP + H(+) + sulfate = adenosine 5'-phosphosulfate + diphosphate; Xref=Rhea:RHEA:18133, ChEBI:CHEBI:15378, ChEBI:CHEBI:16189, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58243; EC=2.7.7.4; Evidence={ECO:0000255|HAMAP- Rule:MF_03106}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.65 mM for Sulfate {ECO:0000269|PubMed:14983089}; KM=0.082 mM for ATP {ECO:0000269|PubMed:14983089}; |
| Pathway: | |
Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. {ECO:0000255|HAMAP-Rule:MF_03106}. |
| Subunit: | |
Homohexamer. Dimer of trimers. {ECO:0000255|HAMAP- Rule:MF_03106, ECO:0000269|PubMed:11157739, ECO:0000269|PubMed:14983089}. |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03106, ECO:0000269|PubMed:14562095}. |
| Induction: | |
Expression depends on the formation of the MET4-MET28-MET31 and MET4-MET28-MET32 complexes on its 5' upstream region. {ECO:0000269|PubMed:9799240}. |
| Domain: | |
The oligomerization domain is distantly related to APS kinases, but it is not functional and does not bind APS. It is required for oligomerization of the enzyme, although the oligomerization state has no effect on the catalytic activity of the enzyme. {ECO:0000255|HAMAP- Rule:MF_03106, ECO:0000269|PubMed:11157739, ECO:0000269|PubMed:14983089}. |
| Miscellaneous: | |
Present with 1510 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the sulfate adenylyltransferase family. {ECO:0000255|HAMAP-Rule:MF_03106}. |
| Sequence caution: | |
Sequence=CAA29702.1; Type=Frameshift; Evidence={ECO:0000305}; Sequence=CAA42726.1; Type=Frameshift; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.