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UniProt functional annotation for P23882 | ||
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| UniProt code: P23882. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Attaches a formyl group to the free amino group of methionyl- tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus. {ECO:0000255|HAMAP-Rule:MF_00182, ECO:0000269|PubMed:6989606, ECO:0000269|PubMed:8331078, ECO:0000269|PubMed:9843487}. |
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| Catalytic activity: | |
Reaction=(6S)-10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N-formyl-L-methionyl- tRNA(fMet); Xref=Rhea:RHEA:24380, Rhea:RHEA-COMP:9952, Rhea:RHEA- COMP:9953, ChEBI:CHEBI:15378, ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:78530, ChEBI:CHEBI:78844; EC=2.1.2.9; Evidence={ECO:0000255|HAMAP-Rule:MF_00182, ECO:0000269|PubMed:6989606, ECO:0000269|PubMed:9843487}; |
| Activity regulation: | |
Activity is optimum in the presence of Mg(2+) and K(+). {ECO:0000269|PubMed:6989606}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=13.5 uM for 10-formyltetrahydrofolate {ECO:0000269|PubMed:6989606}; KM=0.35 uM for L-methionyl-tRNA(fMet) {ECO:0000269|PubMed:6989606}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:6989606}. |
| Domain: | |
Composed of an N- and a C-terminal domain. The N-terminal domain carries the tetrahydrofolate (THF)-binding site and the C- terminal domain is presumably involved in positioning the Met-tRNA substrate for the formylation reaction. {ECO:0000269|PubMed:8887566, ECO:0000269|PubMed:9843487}. |
| Similarity: | |
Belongs to the Fmt family. {ECO:0000255|HAMAP- Rule:MF_00182, ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.