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UniProt functional annotation for P08170 | ||
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| UniProt code: P08170. |
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| Organism: | |
Glycine max (Soybean) (Glycine hispida). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Glycine subgen. Soja. |
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| Function: | |
Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. With linoleate as substrate, L-1 shows a preference for carbon 13 as the site for hydroperoxidation (in contrast to L-2 and L-3, which utilize either carbon 9 or 13). At pH above 8.5, only (9Z,11E,13S)-13- hydroperoxyoctadeca-9,11-dienoate is produced, but as the pH decreases, the proportion of (9S)-hydroperoxide increases linearly until at pH 6.0 it represents about 25 % of the products. {ECO:0000269|PubMed:16157595}. |
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| Catalytic activity: | |
Reaction=(9Z,12Z)-octadecadienoate + O2 = (13S)-hydroperoxy-(9Z,11E)- octadecadienoate; Xref=Rhea:RHEA:22780, ChEBI:CHEBI:15379, ChEBI:CHEBI:30245, ChEBI:CHEBI:57466; EC=1.13.11.12; Evidence={ECO:0000269|PubMed:16157595, ECO:0000269|PubMed:2492826}; |
| Catalytic activity: | |
Reaction=(9Z,12Z,15Z)-octadecatrienoate + O2 = (13S)-hydroperoxy- (9Z,11E,15Z)-octadecatrienoate; Xref=Rhea:RHEA:34495, ChEBI:CHEBI:15379, ChEBI:CHEBI:32387, ChEBI:CHEBI:58757; EC=1.13.11.12; Evidence={ECO:0000269|PubMed:16157595, ECO:0000269|PubMed:2492826}; |
| Catalytic activity: | |
Reaction=(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (17S)- hydroperoxy-(4Z,7Z,10Z,13Z,15E,19Z)-docosahexaenoate; Xref=Rhea:RHEA:50840, ChEBI:CHEBI:15379, ChEBI:CHEBI:77016, ChEBI:CHEBI:133795; Evidence={ECO:0000269|PubMed:19324874}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50841; Evidence={ECO:0000269|PubMed:19324874}; |
| Catalytic activity: | |
Reaction=(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate + O2 = (17S)- hydroperoxy-(4Z,7Z,10Z,13Z,15E)-docosapentaenoate; Xref=Rhea:RHEA:50828, ChEBI:CHEBI:15379, ChEBI:CHEBI:77226, ChEBI:CHEBI:133796; Evidence={ECO:0000269|PubMed:19324874}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50829; Evidence={ECO:0000269|PubMed:19324874}; |
| Catalytic activity: | |
Reaction=(7Z,10Z,13Z,16Z,19Z)-docosapentaenoate + O2 = 17-hydroperoxy- (7Z,10Z,13Z,15E,19Z)-docosapentaenoate; Xref=Rhea:RHEA:50832, ChEBI:CHEBI:15379, ChEBI:CHEBI:77224, ChEBI:CHEBI:133797; Evidence={ECO:0000269|PubMed:19324874}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50833; Evidence={ECO:0000269|PubMed:19324874}; |
| Cofactor: | |
Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000255|PROSITE-ProRule:PRU00726, ECO:0000269|PubMed:8518276}; Note=Binds 1 Fe cation per subunit. Iron is tightly bound. {ECO:0000255|PROSITE-ProRule:PRU00726, ECO:0000269|PubMed:8518276}; |
| Biophysicochemical properties: | |
pH dependence: Inactive below pH 6.0. {ECO:0000269|PubMed:2492826}; |
| Pathway: | |
Lipid metabolism; oxylipin biosynthesis. {ECO:0000255|PROSITE- ProRule:PRU00726}. |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:11412104, ECO:0000269|PubMed:8502991, ECO:0000269|PubMed:8718858}. |
| Subcellular location: | |
Cytoplasm. |
| Induction: | |
The hydroperoxide product serves to activate the resting enzyme. The activation is accompanied by the oxidation of Fe(2+)entadiene structure. L-1 prefers anionic substrate. |
| Miscellaneous: | |
Soybean contains at least 4 distinct isoenzymes, L-1, L- 2, L-3a and L-3b in dry seeds, and at least two distinct isozymes in the hypocotyl/radicle region of the seedling stem. |
| Similarity: | |
Belongs to the lipoxygenase family. {ECO:0000305}. |
| Sequence caution: | |
Sequence=CAA47717.1; Type=Frameshift; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.