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UniProt functional annotation for O04073 | ||
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| UniProt code: O04073. |
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| Organism: | |
Tetradesmus obliquus (Green alga) (Acutodesmus obliquus). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae; CS clade; Sphaeropleales; Scenedesmaceae; Tetradesmus. |
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| Function: | |
Protease involved in the C-terminal processing of the chloroplastic D1 protein of photosystem II. This proteolytic processing is necessary to allow the light-driven assembly of the tetranuclear manganese cluster, which is responsible for photosynthetic water oxidation. {ECO:0000269|PubMed:9252339}. |
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| Catalytic activity: | |
Reaction=The enzyme shows specific recognition of a C-terminal tripeptide, Xaa-Yaa-Zaa, in which Xaa is preferably Ala or Leu, Yaa is preferably Ala or Tyr, and Zaa is preferably Ala, but then cleaves at a variable distance from the C-terminus. A typical cleavage is -Ala-Ala-|-Arg-Ala-Ala-Lys-Glu-Asn-Tyr-Ala-Leu-Ala-Ala.; EC=3.4.21.102; Evidence={ECO:0000303|PubMed:9252339}; |
| Activity regulation: | |
Not inhibited by antipain, 4- amidinophenylmethanesulfonyl fluoride, aprotinin, chymostatin, 3,4- dichloroisocoumarin, diisopropyl fluorophosphate, E64, EDTA, EGTA, iodoacetamide, leupeptin, pepstatin, o-phenanthroline, N- ethylmaleimide, phosphoramidon or phenylmethylsulfonyl fluoride. {ECO:0000269|PubMed:9252339}. |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is 6.2. {ECO:0000269|PubMed:9252339}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:10966643}. |
| Subcellular location: | |
Plastid, chloroplast thylakoid lumen {ECO:0000303|PubMed:9252339}. |
| Disruption phenotype: | |
The LF-1 strain contains a frameshift causing a premature stop codon within ctpA. This strain is unable to process the precursor form of D1. {ECO:0000305|PubMed:9252339}. |
| Similarity: | |
Belongs to the peptidase S41A family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.