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PDBsum entry 1f8q
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Crystal structure of alpha-Momorcharin in 80% acetonitrile--Water mixture.
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Authors
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G.Zhu,
Q.Huang,
M.Qian,
Y.Tang.
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Ref.
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Biochim Biophys Acta, 2001,
1548,
152-158.
[DOI no: ]
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PubMed id
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Abstract
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Crystals of alpha-momorcharin (MMC) were cross-linked and soaked in an 80%
acetonitrile--water mixture and X-ray data were collected to 2.2 A resolution.
MMC is a ribosome-inactivating protein with a sugar chain on Asn-227. In
previous studies, the whole conformation of the sugar chain could not be
obtained in the aqueous system. Here the structure of MMC in a low water system
is shown to be similar to the native one, but the sugar chain on Asn-227 is
defined by the electron density map. Several oxygen atoms of the oligosaccharide
formed intramolecular hydrogen bonds to the protein moiety. The conformation of
the residues in the active center is similar to that in the aqueous system. Our
results show conformational alteration of the tetrasaccharide of MMC in organic
media. They indicate that the oligosaccharides are more rigid in organic
solvents. X-ray determination in organic media may be used to solve some
structures of oligosaccharides linked to glycoproteins.
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Secondary reference #1
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Title
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Studies on crystal structures, Active-Centre geometry and depurinating mechanism of two ribosome-Inactivating proteins.
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Authors
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Q.Huang,
S.Liu,
Y.Tang,
S.Jin,
Y.Wang.
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Ref.
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Biochem J, 1995,
309,
285-298.
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PubMed id
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