UniProt functional annotation for Q55080



	UniProt functional annotation for Q55080

UniProt code: Q55080.

Organism: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770).

Taxonomy: Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; Sulfolobus.

Function: The endogenous substrate is not known. In vitro, catalyzes the H(2)O(2)-dependent epoxidation of styrene, cis-beta-methylstyrene, and cis-stilbene with retention of stereochemistry. Is able to use cumene hydroperoxide (CHP) or tert-butyl hydroperoxide (TBHP) instead of H(2)O(2) as the electron acceptor. Can also hydroxylate fatty acids such as lauric acid. {ECO:0000269|PubMed:10799487, ECO:0000269|PubMed:12010041, ECO:0000269|PubMed:18157853}.

Catalytic activity: Reaction=2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O; Xref=Rhea:RHEA:56136, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:139520, ChEBI:CHEBI:139521; EC=1.11.1.7;

Cofactor: Name=heme; Xref=ChEBI:CHEBI:30413; Note=Binds 1 heme group per subunit.;

Biophysicochemical properties: Kinetic parameters: KM=21 uM for lauric acid {ECO:0000269|PubMed:10799487, ECO:0000269|PubMed:12010041, ECO:0000269|PubMed:18157853}; KM=9.2 mM for styrene {ECO:0000269|PubMed:10799487, ECO:0000269|PubMed:12010041, ECO:0000269|PubMed:18157853}; pH dependence: Optimum pH is 8.5 for peroxidase activity. {ECO:0000269|PubMed:10799487, ECO:0000269|PubMed:12010041, ECO:0000269|PubMed:18157853}; Temperature dependence: Optimum temperature is about 75 degrees Celsius. Activity is 10-fold greater at 75 degrees Celsius than that measured at 25 degrees Celsius. Thermostable up to 85 degrees Celsius. Thermal denaturation midpoint (Tm) is 91 degrees Celsius. {ECO:0000269|PubMed:10799487, ECO:0000269|PubMed:12010041, ECO:0000269|PubMed:18157853};

Subcellular location: Cytoplasm {ECO:0000305}.

Similarity: Belongs to the cytochrome P450 family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770).
Taxonomy:		Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; Sulfolobus.



Function:		The endogenous substrate is not known. In vitro, catalyzes the H(2)O(2)-dependent epoxidation of styrene, cis-beta-methylstyrene, and cis-stilbene with retention of stereochemistry. Is able to use cumene hydroperoxide (CHP) or tert-butyl hydroperoxide (TBHP) instead of H(2)O(2) as the electron acceptor. Can also hydroxylate fatty acids such as lauric acid. {ECO:0000269\|PubMed:10799487, ECO:0000269\|PubMed:12010041, ECO:0000269\|PubMed:18157853}.


Catalytic activity:		Reaction=2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O; Xref=Rhea:RHEA:56136, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:139520, ChEBI:CHEBI:139521; EC=1.11.1.7;
Cofactor:		Name=heme; Xref=ChEBI:CHEBI:30413; Note=Binds 1 heme group per subunit.;
Biophysicochemical properties:		Kinetic parameters: KM=21 uM for lauric acid {ECO:0000269\|PubMed:10799487, ECO:0000269\|PubMed:12010041, ECO:0000269\|PubMed:18157853}; KM=9.2 mM for styrene {ECO:0000269\|PubMed:10799487, ECO:0000269\|PubMed:12010041, ECO:0000269\|PubMed:18157853}; pH dependence: Optimum pH is 8.5 for peroxidase activity. {ECO:0000269\|PubMed:10799487, ECO:0000269\|PubMed:12010041, ECO:0000269\|PubMed:18157853}; Temperature dependence: Optimum temperature is about 75 degrees Celsius. Activity is 10-fold greater at 75 degrees Celsius than that measured at 25 degrees Celsius. Thermostable up to 85 degrees Celsius. Thermal denaturation midpoint (Tm) is 91 degrees Celsius. {ECO:0000269\|PubMed:10799487, ECO:0000269\|PubMed:12010041, ECO:0000269\|PubMed:18157853};
Subcellular location:		Cytoplasm {ECO:0000305}.
Similarity:		Belongs to the cytochrome P450 family. {ECO:0000305}.