UniProt functional annotation for O19069



	UniProt functional annotation for O19069

UniProt code: O19069.

Organism: Sus scrofa (Pig).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.

Function: Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits. {ECO:0000255|HAMAP-Rule:MF_03222}.

Catalytic activity: Reaction=ATP + CoA + succinate = ADP + phosphate + succinyl-CoA; Xref=Rhea:RHEA:17661, ChEBI:CHEBI:30031, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292, ChEBI:CHEBI:456216; EC=6.2.1.5; Evidence={ECO:0000255|HAMAP- Rule:MF_03222};

Catalytic activity: Reaction=CoA + GTP + succinate = GDP + phosphate + succinyl-CoA; Xref=Rhea:RHEA:22120, ChEBI:CHEBI:30031, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292, ChEBI:CHEBI:58189; EC=6.2.1.4; Evidence={ECO:0000255|HAMAP- Rule:MF_03222, ECO:0000269|PubMed:27487822};

Biophysicochemical properties: Kinetic parameters: KM=0.76 mM for succinate {ECO:0000269|PubMed:27487822}; Vmax=5.99 umol/min/mg enzyme {ECO:0000269|PubMed:27487822};

Pathway: Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1. {ECO:0000255|HAMAP- Rule:MF_03222}.

Subunit: Heterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with the ATP- specific beta subunit SUCLA2, forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with the GTP-specific beta subunit SUCLG2 forms a GDP-forming succinyl-CoA synthetase (G-SCS). {ECO:0000255|HAMAP-Rule:MF_03222, ECO:0000269|PubMed:10873456, ECO:0000269|PubMed:16481318, ECO:0000269|PubMed:26249701, ECO:0000269|PubMed:27487822}.

Subcellular location: Mitochondrion {ECO:0000255|HAMAP-Rule:MF_03222}.

Tissue specificity: Isoform I is expressed in all tissues examined: liver, brain, heart, and skeletal muscle. Isoform II is expressed only in heart and skeletal muscle. {ECO:0000269|PubMed:9261120}.

Similarity: Belongs to the succinate/malate CoA ligase alpha subunit family. {ECO:0000255|HAMAP-Rule:MF_03222}.

Sequence caution: Sequence=AAB94003.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=AAB94004.1; Type=Erroneous initiation; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Sus scrofa (Pig).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.



Function:		Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits. {ECO:0000255\|HAMAP-Rule:MF_03222}.


Catalytic activity:		Reaction=ATP + CoA + succinate = ADP + phosphate + succinyl-CoA; Xref=Rhea:RHEA:17661, ChEBI:CHEBI:30031, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292, ChEBI:CHEBI:456216; EC=6.2.1.5; Evidence={ECO:0000255\|HAMAP- Rule:MF_03222};
Catalytic activity:		Reaction=CoA + GTP + succinate = GDP + phosphate + succinyl-CoA; Xref=Rhea:RHEA:22120, ChEBI:CHEBI:30031, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292, ChEBI:CHEBI:58189; EC=6.2.1.4; Evidence={ECO:0000255\|HAMAP- Rule:MF_03222, ECO:0000269\|PubMed:27487822};
Biophysicochemical properties:		Kinetic parameters: KM=0.76 mM for succinate {ECO:0000269\|PubMed:27487822}; Vmax=5.99 umol/min/mg enzyme {ECO:0000269\|PubMed:27487822};
Pathway:		Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1. {ECO:0000255\|HAMAP- Rule:MF_03222}.
Subunit:		Heterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with the ATP- specific beta subunit SUCLA2, forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with the GTP-specific beta subunit SUCLG2 forms a GDP-forming succinyl-CoA synthetase (G-SCS). {ECO:0000255\|HAMAP-Rule:MF_03222, ECO:0000269\|PubMed:10873456, ECO:0000269\|PubMed:16481318, ECO:0000269\|PubMed:26249701, ECO:0000269\|PubMed:27487822}.
Subcellular location:		Mitochondrion {ECO:0000255\|HAMAP-Rule:MF_03222}.
Tissue specificity:		Isoform I is expressed in all tissues examined: liver, brain, heart, and skeletal muscle. Isoform II is expressed only in heart and skeletal muscle. {ECO:0000269\|PubMed:9261120}.
Similarity:		Belongs to the succinate/malate CoA ligase alpha subunit family. {ECO:0000255\|HAMAP-Rule:MF_03222}.
Sequence caution:		Sequence=AAB94003.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=AAB94004.1; Type=Erroneous initiation; Evidence={ECO:0000305};