UniProt functional annotation for Q77DJ6



	UniProt functional annotation for Q77DJ6

UniProt code: Q77DJ6.

Organism: Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus).

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Filoviridae; Ebolavirus.

Function: Plays an essential role virus particle assembly and budding. Acts by interacting with viral ribonucleocapsid and host members of the ESCRT (endosomal sorting complex required for transport) system such as host VPS4, PDCD6IP/ALIX, NEDD4 or TGS101. The interaction with host E3 ubiquitin ligase SMURF2 also facilitates virus budding. May play a role in immune cell dysfunction by being packaged into exosomes that can decrease the viability of recipient cells (via RNAi suppression and exosome-bystander apoptosis). {ECO:0000250|UniProtKB:Q05128}.

Subunit: Homodimer. Homohexamer. Homooctamer. Exists as a dimer until it reorganizes at the plasma membrane into a hexameric form using phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2). Hexamers are critical for budding. Octamers function in genome replication and RNA binding. Interacts with host TSG101. As a homohexamer, interacts with the WW domain 3 of host NEDD4. Interacts with the nucleoprotein/NP. Interacts (via YPx(n)L/I motif) with host PDCD6IP/ALIX; this interaction supports efficient egress of viral particles. Interacts with VP35. Interacts with host ITCH; this interaction is required for efficient egress. Interacts (via PPXY motif) with host SMURF2 (via WW domains); the interaction positively regulates virus budding. {ECO:0000250|UniProtKB:Q05128}.

Subcellular location: Host cytoplasm {ECO:0000250|UniProtKB:Q05128}. Host cell membrane {ECO:0000250|UniProtKB:Q05128}. Virion membrane {ECO:0000250|UniProtKB:P35260}; Peripheral membrane protein {ECO:0000250|UniProtKB:P35260}. Host late endosome membrane {ECO:0000250|UniProtKB:P35260}; Peripheral membrane protein {ECO:0000250|UniProtKB:P35260}. Host cell membrane {ECO:0000250|UniProtKB:P35260}; Peripheral membrane protein {ECO:0000250|UniProtKB:P35260}; Cytoplasmic side {ECO:0000250|UniProtKB:P35260}. Host endomembrane system {ECO:0000250|UniProtKB:P35260}; Peripheral membrane protein {ECO:0000250|UniProtKB:P35260}. Secreted, extracellular exosome {ECO:0000250|UniProtKB:Q05128}. Note=In virion, localizes on the inner side of the membrane. In the host cell, it is found associated with virus-induced membrane proliferation foci and probably also in multivesicular bodies. These VP40-enriched membrane clusters are then redistributed to the plasma membrane where budding takes place. {ECO:0000250|UniProtKB:P35260}.

Domain: Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. VP40 contains two overlapping L domains: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 and a PPXY motif which interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase and the three WW domains of SMURF2 E3 ubiquitin ligase. {ECO:0000250|UniProtKB:Q05128}.

Ptm: Sumoylated with host SUMO1, SUMO2. Sumoylation provides stability to VP40. {ECO:0000250|UniProtKB:Q05128}.

Ptm: Ubiquitinated by host WWP1. This modification mediates efficient viral budding. {ECO:0000250|UniProtKB:Q05128}.

Miscellaneous: Most abundant protein in the virion. {ECO:0000250|UniProtKB:Q05128}.

Similarity: Belongs to the filoviridae matrix protein VP40 family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus).
Taxonomy:		Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Filoviridae; Ebolavirus.



Function:		Plays an essential role virus particle assembly and budding. Acts by interacting with viral ribonucleocapsid and host members of the ESCRT (endosomal sorting complex required for transport) system such as host VPS4, PDCD6IP/ALIX, NEDD4 or TGS101. The interaction with host E3 ubiquitin ligase SMURF2 also facilitates virus budding. May play a role in immune cell dysfunction by being packaged into exosomes that can decrease the viability of recipient cells (via RNAi suppression and exosome-bystander apoptosis). {ECO:0000250\|UniProtKB:Q05128}.


Subunit:		Homodimer. Homohexamer. Homooctamer. Exists as a dimer until it reorganizes at the plasma membrane into a hexameric form using phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2). Hexamers are critical for budding. Octamers function in genome replication and RNA binding. Interacts with host TSG101. As a homohexamer, interacts with the WW domain 3 of host NEDD4. Interacts with the nucleoprotein/NP. Interacts (via YPx(n)L/I motif) with host PDCD6IP/ALIX; this interaction supports efficient egress of viral particles. Interacts with VP35. Interacts with host ITCH; this interaction is required for efficient egress. Interacts (via PPXY motif) with host SMURF2 (via WW domains); the interaction positively regulates virus budding. {ECO:0000250\|UniProtKB:Q05128}.
Subcellular location:		Host cytoplasm {ECO:0000250\|UniProtKB:Q05128}. Host cell membrane {ECO:0000250\|UniProtKB:Q05128}. Virion membrane {ECO:0000250\|UniProtKB:P35260}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P35260}. Host late endosome membrane {ECO:0000250\|UniProtKB:P35260}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P35260}. Host cell membrane {ECO:0000250\|UniProtKB:P35260}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P35260}; Cytoplasmic side {ECO:0000250\|UniProtKB:P35260}. Host endomembrane system {ECO:0000250\|UniProtKB:P35260}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P35260}. Secreted, extracellular exosome {ECO:0000250\|UniProtKB:Q05128}. Note=In virion, localizes on the inner side of the membrane. In the host cell, it is found associated with virus-induced membrane proliferation foci and probably also in multivesicular bodies. These VP40-enriched membrane clusters are then redistributed to the plasma membrane where budding takes place. {ECO:0000250\|UniProtKB:P35260}.
Domain:		Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. VP40 contains two overlapping L domains: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 and a PPXY motif which interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase and the three WW domains of SMURF2 E3 ubiquitin ligase. {ECO:0000250\|UniProtKB:Q05128}.
Ptm:		Sumoylated with host SUMO1, SUMO2. Sumoylation provides stability to VP40. {ECO:0000250\|UniProtKB:Q05128}.
Ptm:		Ubiquitinated by host WWP1. This modification mediates efficient viral budding. {ECO:0000250\|UniProtKB:Q05128}.
Miscellaneous:		Most abundant protein in the virion. {ECO:0000250\|UniProtKB:Q05128}.
Similarity:		Belongs to the filoviridae matrix protein VP40 family. {ECO:0000305}.