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UniProt functional annotation for P29717 | ||
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| UniProt code: P29717. |
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| Organism: | |
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida. |
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| Function: | |
Major glucan 1,3-beta-glucosidase required for cell wall integrity. Beta-glucanases participate in the metabolism of beta- glucan, the main structural component of the cell wall. Can also function biosynthetically as a transglycosylase. Functions to deliver glucan from the cell to the extracellular matrix. Does not appear to impact cell wall glucan content of biofilm cells, nor is it necessary for filamentation or biofilm formation. Involved in cell-substrate and cell-cell adhesion. Adhesion to host-cell surfaces is the first critical step during mucosal infection. XOG1 is target of human antimicrobial peptide LL-37 for inhibition of cell adhesion. {ECO:0000269|PubMed:21713010, ECO:0000269|PubMed:22876186}. |
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| Catalytic activity: | |
Reaction=Successive hydrolysis of beta-D-glucose units from the non- reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose.; EC=3.2.1.58; Evidence={ECO:0000269|PubMed:10469155, ECO:0000269|PubMed:20875088, ECO:0000269|PubMed:21713010}; |
| Activity regulation: | |
Binding of human antimicrobial peptide LL-37 decreases the catalytic activity, which leads to the decrease of cell adhesion. {ECO:0000269|PubMed:21713010}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=23.0 mM for laminaribiose {ECO:0000269|PubMed:10469155}; KM=12.7 mM for laminaritriose {ECO:0000269|PubMed:10469155}; KM=5.0 mM for laminaritetraose {ECO:0000269|PubMed:10469155}; KM=2.6 mM for laminaripentaose {ECO:0000269|PubMed:10469155}; KM=2.7 mM for laminarihexaose {ECO:0000269|PubMed:10469155}; KM=2.4 mM for laminariheptaose {ECO:0000269|PubMed:10469155}; KM=3.9 mM for laminarin {ECO:0000269|PubMed:10469155}; KM=17.9 mM for pustulan {ECO:0000269|PubMed:10469155}; KM=260 mM for gentiobiose {ECO:0000269|PubMed:10469155}; KM=120 mM for gentiotriose {ECO:0000269|PubMed:10469155}; KM=70 mM for cellobiose {ECO:0000269|PubMed:10469155}; KM=9.4 mM for cellotetraose {ECO:0000269|PubMed:10469155}; KM=12.0 mM for cellohexaose {ECO:0000269|PubMed:10469155}; KM=2.4 mM for p-nitrophenyl-beta-glucoside (pNPG) {ECO:0000269|PubMed:10469155}; |
| Subunit: | |
Monomer. Interacts with the human antimicrobial peptide LL-37. {ECO:0000269|PubMed:1789004, ECO:0000269|PubMed:20875088, ECO:0000269|PubMed:21713010}. |
| Subcellular location: | |
Secreted, cell wall {ECO:0000269|PubMed:1789004, ECO:0000269|PubMed:19555771, ECO:0000269|PubMed:20167299, ECO:0000269|PubMed:20641015, ECO:0000269|PubMed:21622905, ECO:0000269|PubMed:23136884, ECO:0000269|PubMed:23243062}. Note=Is non- covalently attached to the cell wall. |
| Induction: | |
Expression is maximal during the early rapid growth phase and increases during biofilm growth. Induced by caspofungin. Transcripts is also regulated by RCK2, SIT4, and HTG4. {ECO:0000269|PubMed:14731272, ECO:0000269|PubMed:17030998, ECO:0000269|PubMed:20392151, ECO:0000269|PubMed:20402792, ECO:0000269|PubMed:21622905, ECO:0000269|PubMed:22876186}. |
| Disruption phenotype: | |
Leads to enhanced susceptibility to the commonly used antifungal, fluconazole, during biofilm growth only. {ECO:0000269|PubMed:22876186}. |
| Similarity: | |
Belongs to the glycosyl hydrolase 5 (cellulase A) family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.