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UniProt functional annotation for P25051 | ||
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| UniProt code: P25051. |
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| Organism: | |
Enterococcus faecium (Streptococcus faecium). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus. |
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| Function: | |
Required for high-level resistance to glycopeptide antibiotics. D-Ala--D-Ala ligase of altered specificity which catalyzes ester bond formation between D-Ala and various D-hydroxy acids; produces a peptidoglycan which does not terminate in D-alanine but in D-lactate, thus preventing vancomycin or teicoplanin binding. {ECO:0000269|PubMed:1931965}. |
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| Catalytic activity: | |
Reaction=(R)-lactate + ATP + D-alanine = ADP + D-alanyl-(R)-lactate + phosphate; Xref=Rhea:RHEA:37347, ChEBI:CHEBI:16004, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57416, ChEBI:CHEBI:61166, ChEBI:CHEBI:456216; EC=6.1.2.1; Evidence={ECO:0000269|PubMed:1931965}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250}; Note=Binds 2 magnesium or manganese ions per subunit. {ECO:0000250}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=7.1 mM for D-lactate {ECO:0000269|PubMed:1931965}; KM=3.8 mM for D-alanine {ECO:0000269|PubMed:1931965}; KM=0.6 mM for 2-hydroxybutyrate {ECO:0000269|PubMed:1931965}; KM=3.2 mM for 2-hydroxyvalerate {ECO:0000269|PubMed:1931965}; KM=11 mM for 2-hydroxycaproate {ECO:0000269|PubMed:1931965}; |
| Subcellular location: | |
Cell membrane; Peripheral membrane protein; Cytoplasmic side. |
| Induction: | |
By vancomycin, mediated by VanS/VanR. |
| Similarity: | |
Belongs to the D-alanine--D-alanine ligase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.