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UniProt functional annotation for Q9QYY9 | ||
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| UniProt code: Q9QYY9. |
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| Organism: | |
Mus musculus (Mouse). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. |
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| Function: | |
Catalyzes the NAD-dependent oxidation of either all-trans- retinol or 9-cis-retinol (By similarity). Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate (PubMed:16081420). Also catalyzes the reduction of benzoquinones (By similarity). {ECO:0000250|UniProtKB:P08319, ECO:0000269|PubMed:16081420}. |
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| Catalytic activity: | |
Reaction=all-trans-retinol + NAD(+) = all-trans-retinal + H(+) + NADH; Xref=Rhea:RHEA:21284, ChEBI:CHEBI:15378, ChEBI:CHEBI:17336, ChEBI:CHEBI:17898, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.105; Evidence={ECO:0000250|UniProtKB:P08319}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:21285; Evidence={ECO:0000250|UniProtKB:P08319}; |
| Catalytic activity: | |
Reaction=20-hydroxy-(5Z,8Z,11Z,14Z)-eicosatetraenoate + NAD(+) = 20- oxo-(5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+) + NADH; Xref=Rhea:RHEA:39799, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:76624, ChEBI:CHEBI:76645; Evidence={ECO:0000269|PubMed:16081420}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:39800; Evidence={ECO:0000269|PubMed:16081420}; |
| Catalytic activity: | |
Reaction=20-oxo-(5Z,8Z,11Z,14Z)-eicosatetraenoate + H2O + NAD(+) = (5Z,8Z,11Z,14Z)-eicosatetraenedioate + 2 H(+) + NADH; Xref=Rhea:RHEA:39803, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:76645, ChEBI:CHEBI:76647; Evidence={ECO:0000269|PubMed:16081420}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:39804; Evidence={ECO:0000269|PubMed:16081420}; |
| Catalytic activity: | |
Reaction=9-cis-retinol + NAD(+) = 9-cis-retinal + H(+) + NADH; Xref=Rhea:RHEA:42052, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:78272, ChEBI:CHEBI:78273; Evidence={ECO:0000250|UniProtKB:P08319}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42053; Evidence={ECO:0000250|UniProtKB:P08319}; |
| Catalytic activity: | |
Reaction=1,4-benzoquinone + H(+) + NADH = hydroquinone + NAD(+); Xref=Rhea:RHEA:60660, ChEBI:CHEBI:15378, ChEBI:CHEBI:16509, ChEBI:CHEBI:17594, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; Evidence={ECO:0000250|UniProtKB:P08319}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60661; Evidence={ECO:0000250|UniProtKB:P08319}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 2 Zn(2+) ions per subunit.; |
| Activity regulation: | |
Oxidation of 20-HETE is inhibited by low concentrations of N-heptylformamide (PubMed:16081420). Oxidation of 20- HETE is a decreased by 55-65% by either all-trans-retinol or all-trans- retinoic acid (PubMed:16081420). Strongly inhibited by omega-hydroxy fatty acids (PubMed:10514444). {ECO:0000269|PubMed:10514444, ECO:0000269|PubMed:16081420}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=35 uM for 20-HETE {ECO:0000269|PubMed:16081420}; KM=0.24 mM for 1,4-benzoquinone {ECO:0000269|PubMed:10514444}; |
| Subunit: | |
Dimer. {ECO:0000269|PubMed:10970744}. |
| Subcellular location: | |
Cytoplasm {ECO:0000250}. |
| Tissue specificity: | |
Liver specific. {ECO:0000269|PubMed:10514444}. |
| Similarity: | |
Belongs to the zinc-containing alcohol dehydrogenase family. Class-II subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.