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UniProt functional annotation for P13000 | ||
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| UniProt code: P13000. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Catalyzes a mechanistically unusual reaction, the ATP- dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8- diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring. Only CTP can partially replace ATP while diaminobiotin is only 37% as effective as 7,8-diaminopelargonic acid (PubMed:4892372, PubMed:4921568). In another study both CTP and GTP (but not ITP, TTP or UTP) can partially replace ATP (PubMed:25801336). {ECO:0000269|PubMed:25801336, ECO:0000269|PubMed:4892372, ECO:0000269|PubMed:4921568}. |
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| Catalytic activity: | |
Reaction=(7R,8S)-7,8-diammoniononanoate + ATP + CO2 = (4R,5S)- dethiobiotin + ADP + 3 H(+) + phosphate; Xref=Rhea:RHEA:15805, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:149469, ChEBI:CHEBI:149473, ChEBI:CHEBI:456216; EC=6.3.3.3; Evidence={ECO:0000255|HAMAP- Rule:MF_00336, ECO:0000269|PubMed:4921568}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_00336, ECO:0000269|PubMed:7669756, ECO:0000269|PubMed:7881906, ECO:0000269|PubMed:9576910, ECO:0000269|PubMed:9865950}; Note=Binds 1 Mg(2+) per subunit, in one structure a second Mg(2+) was seen. {ECO:0000269|PubMed:7669756, ECO:0000269|PubMed:7881906, ECO:0000269|PubMed:9576910, ECO:0000269|PubMed:9865950}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=15.2 uM for DAPA (at pH 7.5, 37 degrees Celsius) {ECO:0000269|PubMed:25801336}; KM=600 uM for NaHCO3 (at pH 7.5, 37 degrees Celsius) {ECO:0000269|PubMed:25801336}; KM=10.5 uM for ATP (at pH 7.5, 37 degrees Celsius) {ECO:0000269|PubMed:25801336}; KM=1.10 mM for CTP (at pH 7.5, 37 degrees Celsius) {ECO:0000269|PubMed:25801336}; KM=1.56 mM for GTP (at pH 7.5, 37 degrees Celsius) {ECO:0000269|PubMed:25801336}; |
| Pathway: | |
Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8- diaminononanoate: step 1/2. {ECO:0000255|HAMAP-Rule:MF_00336}. |
| Subunit: | |
Homodimer. {ECO:0000255|HAMAP-Rule:MF_00336, ECO:0000269|PubMed:4921568, ECO:0000269|PubMed:7669756, ECO:0000269|PubMed:7881906, ECO:0000269|PubMed:8081756, ECO:0000269|PubMed:9576910, ECO:0000269|PubMed:9865950}. |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00336}. |
| Similarity: | |
Belongs to the dethiobiotin synthetase family. {ECO:0000255|HAMAP-Rule:MF_00336}. |
Annotations taken from UniProtKB at the EBI.