PDBsum entry 1dpr

Transcription regulation

PDB id: 1dpr

Contents

Protein chains

134 a.a. *

* Residue conservation analysis

References listed in PDB file

Key reference

• Title: Structures of the apo- And the metal ion-Activated forms of the diphtheria tox repressor from corynebacterium diphtheriae.
• Authors: N.Schiering, X.Tao, H.Zeng, J.R.Murphy, G.A.Petsko, D.Ringe.
• Ref.: Proc Natl Acad Sci U S A, 1995, 92, 9843-9850. [DOI no: 10.1073/pnas.92.21.9843]
• PubMed id: 7568230

Note In the PDB file this reference is annotated as "TO BE PUBLISHED". The citation details given above were identified by an automated search of PubMed on title and author names, giving a percentage match of 92%.

Abstract

The diphtheria tox repressor (DtxR) of Corynebacterium diphtheriae plays a critical role in the regulation of diphtheria toxin expression and the control of other iron-sensitive genes. The crystal structures of apo-DtxR and of the metal ion-activated form of the repressor have been solved and used to identify motifs involved in DNA and metal ion binding. Residues involved in binding of the activated repressor to the diphtheria tox operator, glutamine 43, arginine 47, and arginine 50, were located and confirmed by site-directed mutagenesis. Previous biochemical and genetic data can be explained in terms of these structures. Conformational differences between apo- and Ni-DtxR are discussed with regard to the mechanism of action of this repressor.

Secondary reference #1

• Title: Iron, Dtxr, And the regulation of diphtheria toxin expression.
• Authors: X.Tao, N.Schiering, H.Y.Zeng, D.Ringe, J.R.Murphy.
• Ref.: Mol Microbiol, 1994, 14, 191-197.
• PubMed id: 7830565

Secondary reference #2

• Title: Crystallization and preliminary X-Ray studies of the diphtheria tox repressor from corynebacterium diphtheriae.
• Authors: N.Schiering, X.Tao, J.R.Murphy, G.A.Petsko, D.Ringe.
• Ref.: J Mol Biol, 1994, 244, 654-656.
• PubMed id: 7990147