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PDBsum entry 1dob
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Oxidoreductase
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PDB id
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1dob
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References listed in PDB file
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Key reference
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Title
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The mobile flavin of 4-Oh benzoate hydroxylase.
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Authors
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D.L.Gatti,
B.A.Palfey,
M.S.Lah,
B.Entsch,
V.Massey,
D.P.Ballou,
M.L.Ludwig.
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Ref.
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Science, 1994,
266,
110-114.
[DOI no: ]
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PubMed id
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Note: In the PDB file this reference is
annotated as "TO BE PUBLISHED". The citation details given above were
obtained from the PDBe's
server.
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Abstract
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Para-hydroxybenzoate hydroxylase inserts oxygen into substrates by means of the
labile intermediate, flavin C(4a)-hydroperoxide. This reaction requires
transient isolation of the flavin and substrate from the bulk solvent. Previous
crystal structures have revealed the position of the substrate
para-hydroxybenzoate during oxygenation but not how it enters the active site.
In this study, enzyme structures with the flavin ring displaced relative to the
protein were determined, and it was established that these or similar flavin
conformations also occur in solution. Movement of the flavin appears to be
essential for the translocation of substrates and products into the
solvent-shielded active site during catalysis.
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Secondary reference #1
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Title
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Structures of mutant p-Hydroxybenzoate hydroxylases: evidence for an alternative mode of flavin binding
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Authors
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M.S.Lah,
D.Gatti,
H.A.Schreuder,
B.A.Palfey,
M.L.Ludwig.
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Ref.
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TO BE PUBLISHED ...
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