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UniProt functional annotation for P00914 | ||
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| UniProt code: P00914. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation. |
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| Catalytic activity: | |
Reaction=Cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA).; EC=4.1.99.3; |
| Cofactor: | |
Name=FAD; Xref=ChEBI:CHEBI:57692; Note=Binds 1 FAD per subunit.; |
| Cofactor: | |
Name=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate; Xref=ChEBI:CHEBI:15636; Note=Binds 1 5,10-methenyltetrahydrofolate (MTHF) non-covalently per subunit.; |
| Biophysicochemical properties: | |
Absorption: Abs(max)=384 nm; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:7604260}. |
| Miscellaneous: | |
There are only 10-20 molecules of photolyase per E.coli cell. |
| Miscellaneous: | |
Upon absorption of visible light electrons are transferred from Trp-307 through Trp-360 to Trp 383, and from there to FADH, giving rise to the fully reduced catalytic FADH(-). |
| Similarity: | |
Belongs to the DNA photolyase class-1 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.