|
|
||
|
|
|
|
|
UniProt functional annotation for P00459 | ||
|
|
|
|
|
|
||
| UniProt code: P00459. |
|
||
| Organism: | |
Azotobacter vinelandii. |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Azotobacter. |
|
||
|
||
| Function: | |
The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein (component 2) and a component 1 which is either a molybdenum- iron protein, a vanadium-iron, or an iron-iron protein. |
|
||
| Catalytic activity: | |
Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA- COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997, ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=1.18.6.1; |
| Cofactor: | |
Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster per dimer.; |
| Activity regulation: | |
Nitrogenase holoenzyme is subject to 'conformational protection' by FeSII; under oxidizing conditions FeSII binds to the holoenzyme and reversibly protects it from oxidation (PubMed:7830548). {ECO:0000269|PubMed:7830548}. |
| Subunit: | |
Homodimer. |
| Induction: | |
Constitutively expressed during log and stationary phase in sucrose-limited cultures, its levels decrease during stationary phase (at protein level). {ECO:0000269|PubMed:7830548}. |
| Ptm: | |
The reversible ADP-ribosylation of Arg-101 inactivates the nitrogenase reductase and regulates nitrogenase activity. {ECO:0000250}. |
| Miscellaneous: | |
This subunit is associated with the molybdenum-iron nitrogenase component 2. |
| Similarity: | |
Belongs to the NifH/BchL/ChlL family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.