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PDBsum entry 1dcc
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Oxidoreductase(h2o2(a))
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PDB id
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1dcc
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References listed in PDB file
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Key reference
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Title
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2.2 a structure of oxy-Peroxidase as a model for the transient enzyme: peroxide complex.
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Authors
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M.A.Miller,
A.Shaw,
J.Kraut.
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Ref.
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Nat Struct Biol, 1994,
1,
524-531.
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PubMed id
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Abstract
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The Fe+3-OOH complex of peroxidases has a very short half life, and its
structure cannot be determined by conventional methods. The Fe+2-O2 complex
provides a useful structural model for this intermediate, as it differs by only
one electron and one proton from the transient Fe+3-OOH complex. We therefore
determined the crystal structure of the Fe+2-O2 complex formed by a yeast
cytochrome c peroxidase mutant with Trp 191 replaced by Phe. The refined
structure shows that dioxygen can form a hydrogen bond with the conserved distal
His residue, but not with the conserved distal Arg residue. When the transient
Fe+3-OOH complex is modelled in a similar orientation, the active site of
peroxidase appears to be optimized for catalysing proton transfer between the
vicinal oxygen atoms of the peroxy-anion.
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Secondary reference #1
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Title
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Reaction of ferrous cytochrome c peroxidase with dioxygen: site-Directed mutagenesis provides evidence for rapid reduction of dioxygen by intramolecular electron transfer from the compound i radical site.
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Authors
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M.A.Miller,
D.Bandyopadhyay,
J.M.Mauro,
T.G.Traylor,
J.Kraut.
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Ref.
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Biochemistry, 1992,
31,
2789-2797.
[DOI no: ]
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PubMed id
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Secondary reference #2
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Title
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X-Ray structures of recombinant yeast cytochrome c peroxidase and three heme-Cleft mutants prepared by site-Directed mutagenesis.
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Authors
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J.M.Wang,
M.Mauro,
S.L.Edwards,
S.J.Oatley,
L.A.Fishel,
V.A.Ashford,
N.H.Xuong,
J.Kraut.
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Ref.
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Biochemistry, 1990,
29,
7160-7173.
[DOI no: ]
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PubMed id
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Secondary reference #3
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Title
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Crystal structure of yeast cytochrome c peroxidase refined at 1.7-A resolution.
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Authors
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B.C.Finzel,
T.L.Poulos,
J.Kraut.
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Ref.
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J Biol Chem, 1984,
259,
13027-13036.
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PubMed id
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