 |
PDBsum entry 1d7e
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Photosynthesis
|
PDB id
|
|
|
|
1d7e
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
|
|
* Residue conservation analysis
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
Conformational substates in different crystal forms of the photoactive yellow protein--Correlation with theoretical and experimental flexibility.
|
|
|
Authors
|
|
D.M.Van aalten,
W.Crielaard,
K.J.Hellingwerf,
L.Joshua-Tor.
|
|
|
Ref.
|
|
Protein Sci, 2000,
9,
64-72.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
The conformational changes during the photocycle of the photoactive yellow
protein have been the subject of many recent studies. Spectroscopic measurements
have shown that the photocycle also occurs in a crystalline environment, and
this has been the basis for subsequent Laue diffraction and cryocrystallographic
studies. These studies have shown that conformational changes during the
photocycle are limited to the chromophore and its immediate environment.
However, spectroscopic studies suggest the presence of large conformational
changes in the protein. Here, we address this apparent discrepancy in two ways.
First, we obtain a description of large concerted motions in the ground state of
the yellow protein from NMR data and theoretical calculations. Second, we
describe the high-resolution structure of the yellow protein crystallized in a
different space group. The structure of the yellow protein differs significantly
between the two crystal forms. We show that these differences can be used to
obtain a description of the flexibility of the protein that is consistent with
the motions observed in solution.
|
|
|
Secondary reference #1
|
|
|
Title
|
|
1.4 a structure of photoactive yellow protein, A cytosolic photoreceptor: unusual fold, Active site, And chromophore.
|
|
|
Authors
|
|
G.E.Borgstahl,
D.R.Williams,
E.D.Getzoff.
|
|
|
Ref.
|
|
Biochemistry, 1995,
34,
6278-6287.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
Secondary reference #2
|
|
|
Title
|
|
Solution structure and backbone dynamics of the photoactive yellow protein.
|
|
|
Authors
|
|
P.DüX,
G.Rubinstenn,
G.W.Vuister,
R.Boelens,
F.A.Mulder,
K.Hård,
W.D.Hoff,
A.R.Kroon,
W.Crielaard,
K.J.Hellingwerf,
R.Kaptein.
|
|
|
Ref.
|
|
Biochemistry, 1998,
37,
12689-12699.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
|
|
|
|
