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UniProt functional annotation for P30074 | ||
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| UniProt code: P30074. |
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| Organism: | |
Medicago sativa (Alfalfa). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago. |
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| Function: | |
The primary product of this enzyme is 4,2',4',6'- tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin. {ECO:0000269|PubMed:10653632, ECO:0000269|PubMed:11732902, ECO:0000269|PubMed:11959984, ECO:0000269|PubMed:15380179}. |
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| Catalytic activity: | |
Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'- tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74; Evidence={ECO:0000255|PROSITE-ProRule:PRU10023}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=4.1 uM for malonyl-CoA {ECO:0000269|PubMed:10653632, ECO:0000269|PubMed:11732902, ECO:0000269|PubMed:11959984}; KM=4.5 uM for 4-coumaroyl-CoA {ECO:0000269|PubMed:10653632, ECO:0000269|PubMed:11732902, ECO:0000269|PubMed:11959984}; KM=2.2 uM for benzoyl-CoA {ECO:0000269|PubMed:10653632, ECO:0000269|PubMed:11732902, ECO:0000269|PubMed:11959984}; KM=4.1 uM for hexanoyl-CoA {ECO:0000269|PubMed:10653632, ECO:0000269|PubMed:11732902, ECO:0000269|PubMed:11959984}; KM=5.1 uM for phenylacetyl-CoA {ECO:0000269|PubMed:10653632, ECO:0000269|PubMed:11732902, ECO:0000269|PubMed:11959984}; KM=5.2 uM for feruloyl-CoA {ECO:0000269|PubMed:10653632, ECO:0000269|PubMed:11732902, ECO:0000269|PubMed:11959984}; |
| Pathway: | |
Secondary metabolite biosynthesis; flavonoid biosynthesis. |
| Subunit: | |
Homodimer. {ECO:0000305|PubMed:10426957, ECO:0000305|PubMed:10653632}. |
| Similarity: | |
Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.