|
|
||
|
|
|
|
|
UniProt functional annotation for Q07841 | ||
|
|
|
|
|
|
||
| UniProt code: Q07841. |
|
||
| Organism: | |
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui). |
| Taxonomy: | |
Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Haloarculaceae; Haloarcula. |
|
||
|
||
| Function: | |
Catalyzes the reversible oxidation of malate to oxaloacetate. {ECO:0000269|PubMed:8476859}. |
|
||
| Catalytic activity: | |
Reaction=(S)-malate + NAD(+) = H(+) + NADH + oxaloacetate; Xref=Rhea:RHEA:21432, ChEBI:CHEBI:15378, ChEBI:CHEBI:15589, ChEBI:CHEBI:16452, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.37; Evidence={ECO:0000269|PubMed:8476859}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.9 mM for oxaloacetate (in the presence of 2 M NaCl) {ECO:0000269|PubMed:8476859}; KM=0.7 mM for oxaloacetate (in the presence of 4 M NaCl) {ECO:0000269|PubMed:8476859}; Note=kcat is 197 sec(-1) with oxaloacetate as substrate (in the presence of 2 M NaCl). kcat is 69 sec(-1) with oxaloacetate as substrate (in the presence of 4 M NaCl).; |
| Subunit: | |
Homotetramer; arranged as a dimer of dimers. {ECO:0000269|PubMed:10653643, ECO:0000269|PubMed:10653644, ECO:0000269|PubMed:12581646}. |
| Subcellular location: | |
Cytoplasm {ECO:0000269|PubMed:8476859}. |
| Miscellaneous: | |
The quaternary structure is stabilized by chloride ions bound between the subunits. This may be an adaptation to the halophilic environment. |
| Similarity: | |
Belongs to the LDH/MDH superfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.