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PDBsum entry 1d1h
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Solution structure of hanatoxin1, A gating modifier of voltage-Dependent k(+) channels: common surface features of gating modifier toxins.
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Authors
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H.Takahashi,
J.I.Kim,
H.J.Min,
K.Sato,
K.J.Swartz,
I.Shimada.
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Ref.
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J Mol Biol, 2000,
297,
771-780.
[DOI no: ]
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PubMed id
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Abstract
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The three-dimensional structure of hanatoxin1 (HaTx1) was determined by using
NMR spectroscopy. HaTx1 is a 35 amino acid residue peptide toxin that inhibits
the drk1 voltage-gated K(+) channel not by blocking the pore, but by altering
the energetics of gating. Both the amino acid sequence of HaTx1 and its unique
mechanism of action distinguish this toxin from the previously described K(+)
channel inhibitors. Unlike most other K(+) channel-blocking toxins, HaTx1 adopts
an "inhibitor cystine knot" motif and is composed of two beta-strands,
strand I for residues 19-21 and strand II for residues 28-30, connected by four
chain reversals. A comparison of the surface features of HaTx1 with those of
other gating modifier toxins of voltage-gated Ca(2+) and Na(+) channels suggests
that the combination of a hydrophobic patch and surrounding charged residues is
principally responsible for the binding of gating modifier toxins to
voltage-gated ion channels.
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Figure 6.
Figure 6. (a) Surface profile of HaTx1. (b) Stereo view of the backbone and side-chain heavy atoms of HaTx1. In
(a) and (b), hydrophobic residues (Ala, Cys, Ile, Leu, Met, Phe, Pro, Trp, Tyr and Val) are green; basic (Arg and Lys)
and acidic (Asp and Glu) residues are blue and red, respectively. The residues in the surface hydrophobic patch and
the surrounding charged residues are labeled.
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Figure 7.
Figure 7. Comparison of surface profile between (a)
CsE-V (PDB code 1NRA) and (b) ATX III (PDB code
1ANS). The color code is the same as that of Figure 6.
The residues which belong to a hydrophobic patch and
surrounding basic residues are labeled.
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The above figures are
reprinted
by permission from Elsevier:
J Mol Biol
(2000,
297,
771-780)
copyright 2000.
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Secondary reference #1
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Title
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An inhibitor of the kv2.1 potassium channel isolated from the venom of a chilean tarantula.
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Authors
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K.J.Swartz,
R.Mackinnon.
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Ref.
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Neuron, 1995,
15,
941-949.
[DOI no: ]
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PubMed id
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