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PDBsum entry 1d1f
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References listed in PDB file
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Key reference
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Title
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Stabilization of the helical structure of y2-Selective analogues of neuropeptide y by lactam bridges.
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Authors
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S.Yao,
M.A.Smith-White,
E.K.Potter,
R.S.Norton.
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Ref.
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J Med Chem, 2002,
45,
2310-2318.
[DOI no: ]
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PubMed id
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Abstract
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The importance of helical structure in an analogue of NPY selective for the Y2
receptor, Ac[Leu28,31]NPY24-36, has been investigated by introducing a lactam
bridge between positions 28 and 32. The resulting analogue,
Ac-cyclo28/32[Ala24,Lys28,Leu31,Glu32]NPY24-36, is a potent Y2-selective
agonist. Structural analysis by NMR shows that this analogue forms a helical
structure in a 40% trifluoroethanol/water mixture, whereas in water only the
region around the lactam bridge (Lys28-Glu32) adopts helical-like structure,
with both N- and C-termini being poorly defined. The observation of well-defined
helical structure in aqueous TFE contrasts with that reported for a similar
analogue, Ac-cyclo28/32[Lys28,Glu32]NPY25-36 (Rist et al. FEBS Lett. 1996, 394,
169-173), which consisted of a hairpin-like structure that brought the N- and
C-termini into proximity. We have therefore determined the structures of this
analogue, as well as those of Ac-cyclo28/32[Ala24,Lys28,Leu31,Glu32]NPY24-36 and
Ac-cyclo28/32[Ala24,Lys28,Glu32]NPY24-36, under identical solution conditions
(30% TFE/H2O mixture at 308 K) and find essentially the same helical structure
in all three peptides. These findings support the proposal that these
Y2-selective analogues adopt a helical structure when bound to the Y2 receptor.
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Secondary reference #1
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Title
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Helical structure and self-Association in a 13 residue neuropeptide y y2 receptor agonist: relationship to biological activity.
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Authors
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K.J.Barnham,
F.Catalfamo,
P.K.Pallaghy,
G.J.Howlett,
R.S.Norton.
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Ref.
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Biochim Biophys Acta, 1999,
1435,
127-137.
[DOI no: ]
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PubMed id
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Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
percentage match of
91%.
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Secondary reference #2
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Title
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Solution structure of human neuropeptide y.
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Authors
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S.A.Monks,
G.Karagianis,
G.J.Howlett,
R.S.Norton.
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Ref.
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J Biomol Nmr, 1996,
8,
379-390.
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PubMed id
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