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PDBsum entry 1d0n
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Contractile protein
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PDB id
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1d0n
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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The crystal structure of plasma gelsolin: implications for actin severing, Capping, And nucleation.
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Authors
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L.D.Burtnick,
E.K.Koepf,
J.Grimes,
E.Y.Jones,
D.I.Stuart,
P.J.Mclaughlin,
R.C.Robinson.
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Ref.
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Cell, 1997,
90,
661-670.
[DOI no: ]
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PubMed id
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Abstract
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The structure of gelsolin has been determined by crystallography and comprises
six structurally related domains that, in a Ca2+-free environment, pack together
to form a compact globular structure in which the putative actin-binding
sequences are not sufficiently exposed to enable binding to occur. We propose
that binding Ca2+ can release the connections that join the N- and C-terminal
halves of gelsolin, enabling each half to bind actin relatively independently.
Domain shifts are proposed in response to Ca2+ as bases for models of how
gelsolin acts to sever, cap, or nucleate F-actin filaments. The structure also
invites discussion of polyphosphoinositide binding to segment 2 and suggests how
mutation at Asp-187 could initiate a series of events that lead to deposition of
amyloid plaques, as observed in victims of familial amyloidosis (Finnish type).
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Figure 1.
Figure 1. The Structure of Gelsolin(A) Schematic
representations of the six domains of gelsolin in similar
orientations. A standard coloring of the segments is used here
and in subsequent figures: S1, red; S2, light green; S3, yellow;
S4, pink; S5, dark green; and S6, orange.(B) The relative
organization of S1–S3 and S4–S6. The schematic
representation shows S1–S3 (upper panel) and S4–S6 (lower
panel) in approximately the same orientation. Linker regions are
striped with the colors of the linked segments.(C) Schematic
representations of the structure of whole gelsolin. The upper
panel shows a view rotated by 90° clockwise around the
horizontal with respect to the lower panel. The C-terminal
extension is striped orange and black.
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Figure 2.
Figure 2. The Topology of GelsolinThe topology of gelsolin.
β strands are depicted as arrows and helices as rectangles.
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The above figures are
reprinted
by permission from Cell Press:
Cell
(1997,
90,
661-670)
copyright 1997.
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