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PDBsum entry 1cus
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Hydrolase(serine esterase)
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PDB id
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1cus
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References listed in PDB file
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Key reference
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Title
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Fusarium solani cutinase is a lipolytic enzyme with a catalytic serine accessible to solvent.
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Authors
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C.Martinez,
P.De geus,
M.Lauwereys,
G.Matthyssens,
C.Cambillau.
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Ref.
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Nature, 1992,
356,
615-618.
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PubMed id
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Abstract
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Lipases belong to a class of esterases whose activity on triglycerides is
greatly enhanced at lipid-water interfaces. This phenomenon, called interfacial
activation, has a structural explanation: a hydrophobic lid, which at rest
covers the catalytic site, is displaced on substrate or inhibitor binding and
probably interacts with the lipid matrix. Fusarium solani pisi cutinase belongs
to a group of homologous enzymes of relative molecular mass 22-25K (ref. 7)
capable of degrading cutin, the insoluble lipid-polyester matrix covering the
surface of plants, and hydrolysing triglycerides. Cutinases differ from
classical lipases in that they do not exhibit interfacial activation; they are
active on soluble as well as on emulsified triglycerides. Cutinases therefore
establish a bridge between esterases and lipases. We report here the
three-dimensional structure of a recombinant cutinase from F. solani pisi,
expressed in Escherichia coli. Cutinase is an alpha-beta protein; the active
site is composed of the triad Ser 120, His 188 and Asp 175. Unlike other
lipases, the catalytic serine is not buried under surface loops, but is
accessible to solvent. This could explain why cutinase does not display
interfacial activation.
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Secondary reference #1
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Title
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Cutinase, A lipolytic enzyme with a preformed oxyanion hole.
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Authors
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C.Martinez,
A.Nicolas,
H.Van tilbeurgh,
M.P.Egloff,
C.Cudrey,
R.Verger,
C.Cambillau.
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Ref.
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Biochemistry, 1994,
33,
83-89.
[DOI no: ]
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PubMed id
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Secondary reference #2
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Title
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Engineering cysteine mutants to obtain crystallographic phases with a cutinase from fusarium solani pisi.
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Authors
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C.Martinez,
P.De geus,
P.Stanssens,
M.Lauwereys,
C.Cambillau.
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Ref.
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Protein Eng, 1993,
6,
157-165.
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PubMed id
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Secondary reference #3
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Title
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Crystallization and preliminary X-Ray study of a recombinant cutinase from fusarium solani pisi.
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Authors
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C.Abergel,
C.Martinez,
J.Fontecilla-Camps,
C.Cambillau,
P.De geus,
M.Lauwereys.
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Ref.
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J Mol Biol, 1990,
215,
215-216.
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PubMed id
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