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PDBsum entry 1csp
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Transcription regulation
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PDB id
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1csp
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References listed in PDB file
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Key reference
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Title
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Universal nucleic acid-Binding domain revealed by crystal structure of the b. Subtilis major cold-Shock protein.
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Authors
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H.Schindelin,
M.A.Marahiel,
U.Heinemann.
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Ref.
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Nature, 1993,
364,
164-168.
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PubMed id
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Abstract
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The cold-shock response in both Escherichia coli and Bacillus subtilis is
induced by an abrupt downshift in growth temperature. It leads to the increased
production of the major cold-shock proteins, CS7.4 and CspB, respectively. CS7.4
is a transcriptional activator of two genes. CS7.4 and CspB share 43 per cent
sequence identity with the nucleic acid-binding domain of the eukaryotic
gene-regulatory Y-box factors. This cold-shock domain is conserved from bacteria
to man and contains the RNA-binding RNP1 sequence motif. As a prototype of the
cold-shock domain, the structure of CspB has been determined here from two
crystal forms. In both, CspB is present as an antiparallel five-stranded
beta-barrel. Three consecutive beta-strands, the central one containing the RNP1
motif, create a surface rich in aromatic and basic residues that are presumably
involved in nucleic acid binding. Preferential binding of CspB to
single-stranded DNA is observed in gel retardation experiments.
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Secondary reference #1
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Title
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Overproduction, Crystallization, And preliminary X-Ray diffraction studies of the major cold shock protein from bacillus subtilis, Cspb.
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Authors
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H.Schindelin,
M.Herrler,
G.Willimsky,
M.A.Marahiel,
U.Heinemann.
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Ref.
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Proteins, 1992,
14,
120-124.
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PubMed id
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