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PDBsum entry 1cpp
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Oxidoreductase(oxygenase)
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PDB id
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1cpp
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References listed in PDB file
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Key reference
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Title
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The 2.6-A crystal structure of pseudomonas putida cytochrome p-450.
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Authors
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T.L.Poulos,
B.C.Finzel,
I.C.Gunsalus,
G.C.Wagner,
J.Kraut.
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Ref.
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J Biol Chem, 1985,
260,
16122-16130.
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PubMed id
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Abstract
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The crystal structure of Pseudomonas putida cytochrome P-450cam in the ferric,
camphor bound form has been determined and partially refined to R = 0.23 at 2.6
A. The single 414 amino acid polypeptide chain (Mr = 45,000) approximates a
triangular prism with a maximum dimension of approximately 60 A and a minimum of
approximately 30 A. Twelve helical segments (A through L) account for
approximately 40% of the structure while antiparallel beta pairs account for
only approximately 10%. The unexposed iron protoporphyrin IX is sandwiched
between two parallel helices designated the proximal and distal helices. The
heme iron atom is pentacoordinate with the axial sulfur ligand provided by Cys
357 which extends from the N-terminal end of the proximal (L) helix. A substrate
molecule, 2-bornanone (camphor), is buried in an internal pocket just above the
heme distal surface adjacent to the oxygen binding site. The substrate molecule
is held in place by a hydrogen bond between the side chain hydroxyl group of Tyr
96 and the camphor carbonyl oxygen atom in addition to complementary hydrophobic
contacts between the camphor molecule and neighboring aliphatic and aromatic
residues. The camphor is oriented such that the exo-surface of C5 would contact
an iron bound, "activated" oxygen atom for stereoselective hydroxylation.
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Secondary reference #1
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Title
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Crystal structure of substrate-Free pseudomonas putida cytochrome p-450.
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Authors
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T.L.Poulos,
B.C.Finzel,
A.J.Howard.
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Ref.
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Biochemistry, 1986,
25,
5314-5322.
[DOI no: ]
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PubMed id
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Secondary reference #2
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Title
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Heme enzyme structure and function
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Authors
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T.L.Poulos,
B.C.Finzel.
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Ref.
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pept protein rev, 1984,
4,
115.
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Secondary reference #3
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Title
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Preliminary crystallographic data on cytochrome p-450cam.
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Authors
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T.L.Poulos,
M.Perez,
G.C.Wagner.
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Ref.
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J Biol Chem, 1982,
257,
10427-10429.
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PubMed id
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Secondary reference #4
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Title
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The primary structure of the monoxygenase cytochrome p450cam.
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Authors
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M.Haniu,
L.G.Armes,
M.Tanaka,
K.T.Yasunobu,
B.S.Shastry,
G.C.Wagner,
I.C.Gunsalus.
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Ref.
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Biochem Biophys Res Commun, 1982,
105,
889-894.
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PubMed id
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Headers
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