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PDBsum entry 1clz
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Immunoglobulin
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PDB id
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1clz
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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The X-Ray structure of an anti-Tumour antibody in complex with antigen.
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Authors
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P.D.Jeffrey,
J.Bajorath,
C.Y.Chang,
D.Yelton,
I.Hellström,
K.E.Hellström,
S.Sheriff.
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Ref.
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Nat Struct Biol, 1995,
2,
466-471.
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PubMed id
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Abstract
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The crystal structures of the murine BR96 Fab and its human chimera have been
determined in complex with the nonoate methyl ester derivative of Lewis Y (nLey)
at 2.8 A and 2.5 A resolution, respectively. BR96 binds the carbohydrate in a
large pocket which is formed by residues of all CDR loops except L2. The binding
of the carbohydrate is mediated predominantly by aromatic residues in BR96.
Analysis of the structure suggests that BR96 is capable of recognizing a
structure larger than the Le(y) tetrasaccharide, providing a possible
explanation for its high tumour selectivity. The structure provides a rationale
for mutagenesis experiments that have resulted in BR96 CDR loop mutants with
increased affinity for nLey and/or tumour cells.
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Secondary reference #1
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Title
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Crystallization and preliminary X-Ray analysis of the monoclonal anti-Tumor antibody br96 and its complex with the lewis y determinant.
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Authors
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C.Y.Chang,
P.D.Jeffrey,
J.Bajorath,
I.Hellström,
K.E.Hellström,
S.Sheriff.
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Ref.
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J Mol Biol, 1994,
235,
372-376.
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PubMed id
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