UniProt functional annotation for P27548



	UniProt functional annotation for P27548

UniProt code: P27548.

Organism: Mus musculus (Mouse).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.

Function: Cytokine that acts as a ligand to CD40/TNFRSF5 (By similarity). Costimulates T-cell proliferation and cytokine production (By similarity). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (By similarity). Induces the activation of NF-kappa-B (By similarity). Induces the activation of kinases MAPK8 and PAK2 in T-cells (By similarity). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (PubMed:1374165). Involved in immunoglobulin class switching (PubMed:1374165). {ECO:0000250|UniProtKB:P29965, ECO:0000269|PubMed:1374165}.

Function: [CD40 ligand, soluble form]: Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling. {ECO:0000250|UniProtKB:P29965}.

Subunit: Homotrimer (PubMed:8095800). Interacts with CD28 (By similarity). CD40 ligand, soluble form: Exists as either a monomer or a homotrimer (By similarity). Forms a ternary complex between CD40 and integrins for CD40-CD40LG signaling (By similarity). {ECO:0000250|UniProtKB:P29965, ECO:0000303|PubMed:8095800}.

Subcellular location: Cell membrane {ECO:0000250|UniProtKB:P29965}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P29965}. Cell surface {ECO:0000250|UniProtKB:P29965}.

Subcellular location: [CD40 ligand, soluble form]: Secreted {ECO:0000250|UniProtKB:P29965}. Note=Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and a matrix metalloproteinases (MMP) inhibitor-sensitive pathway. {ECO:0000250|UniProtKB:P29965}.

Tissue specificity: Specifically expressed on activated CD4+ T- lymphocytes.

Ptm: The soluble form derives from the membrane form by proteolytic processing. {ECO:0000250|UniProtKB:P29965}.

Similarity: Belongs to the tumor necrosis factor family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Mus musculus (Mouse).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.



Function:		Cytokine that acts as a ligand to CD40/TNFRSF5 (By similarity). Costimulates T-cell proliferation and cytokine production (By similarity). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (By similarity). Induces the activation of NF-kappa-B (By similarity). Induces the activation of kinases MAPK8 and PAK2 in T-cells (By similarity). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (PubMed:1374165). Involved in immunoglobulin class switching (PubMed:1374165). {ECO:0000250\|UniProtKB:P29965, ECO:0000269\|PubMed:1374165}.



Function:		[CD40 ligand, soluble form]: Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling. {ECO:0000250\|UniProtKB:P29965}.


Subunit:		Homotrimer (PubMed:8095800). Interacts with CD28 (By similarity). CD40 ligand, soluble form: Exists as either a monomer or a homotrimer (By similarity). Forms a ternary complex between CD40 and integrins for CD40-CD40LG signaling (By similarity). {ECO:0000250\|UniProtKB:P29965, ECO:0000303\|PubMed:8095800}.
Subcellular location:		Cell membrane {ECO:0000250\|UniProtKB:P29965}; Single-pass type II membrane protein {ECO:0000250\|UniProtKB:P29965}. Cell surface {ECO:0000250\|UniProtKB:P29965}.
Subcellular location:		[CD40 ligand, soluble form]: Secreted {ECO:0000250\|UniProtKB:P29965}. Note=Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and a matrix metalloproteinases (MMP) inhibitor-sensitive pathway. {ECO:0000250\|UniProtKB:P29965}.
Tissue specificity:		Specifically expressed on activated CD4+ T- lymphocytes.
Ptm:		The soluble form derives from the membrane form by proteolytic processing. {ECO:0000250\|UniProtKB:P29965}.
Similarity:		Belongs to the tumor necrosis factor family. {ECO:0000305}.