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UniProt functional annotation for P80078 | ||
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| UniProt code: P80078. |
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| Organism: | |
Clostridium cochlearium. |
| Taxonomy: | |
Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium. |
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| Function: | |
Catalyzes the carbon skeleton rearrangement of L-glutamate to L-threo-3-methylaspartate ((2S,3S)-3-methylaspartate). {ECO:0000255|HAMAP-Rule:MF_00526, ECO:0000269|PubMed:1315276, ECO:0000269|PubMed:7880251, ECO:0000269|PubMed:8013871}. |
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| Catalytic activity: | |
Reaction=(2S,3S)-3-methyl-L-aspartate = L-glutamate; Xref=Rhea:RHEA:12857, ChEBI:CHEBI:29985, ChEBI:CHEBI:58724; EC=5.4.99.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00526, ECO:0000269|PubMed:7880251, ECO:0000269|PubMed:8013871}; |
| Cofactor: | |
Name=adenosylcob(III)alamin; Xref=ChEBI:CHEBI:18408; Evidence={ECO:0000255|HAMAP-Rule:MF_00526, ECO:0000269|PubMed:10467146, ECO:0000269|PubMed:11592143, ECO:0000269|PubMed:1315276, ECO:0000269|PubMed:7880251, ECO:0000269|PubMed:8013871}; |
| Activity regulation: | |
Competitively inhibited by (2S,4S)-4- fluoroglutamate, 2-methyleneglutarate, (2R,3RS)-3-fluoroglutamate and (S)-3-methylitaconate. {ECO:0000269|PubMed:1315276, ECO:0000269|PubMed:7880251}. |
| Pathway: | |
Amino-acid degradation; L-glutamate degradation via mesaconate pathway; acetate and pyruvate from L-glutamate: step 1/4. {ECO:0000255|HAMAP-Rule:MF_00526}. |
| Subunit: | |
Heterotetramer composed of 2 epsilon subunits (GlmE) and 2 sigma subunits (GlmS). GlmE exists as a homodimer and GlmS as a monomer. {ECO:0000255|HAMAP-Rule:MF_00526, ECO:0000269|PubMed:10467146, ECO:0000269|PubMed:11592143, ECO:0000269|PubMed:1315276, ECO:0000269|PubMed:7880251, ECO:0000269|PubMed:8013871}. |
| Similarity: | |
Belongs to the methylaspartate mutase GlmS subunit family. {ECO:0000255|HAMAP-Rule:MF_00526}. |
Annotations taken from UniProtKB at the EBI.