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PDBsum entry 1cau
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Seed storage protein
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PDB id
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1cau
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Determination of three crystal structures of canavalin by molecular replacement.
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Authors
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T.P.Ko,
J.D.Ng,
J.Day,
A.Greenwood,
A.Mcpherson.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 1993,
49,
478-489.
[DOI no: ]
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PubMed id
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Abstract
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Canavalin, the major reserve protein of the jack bean, was obtained in four
different crystal forms. From the structure determined by multiple isomorphous
replacement in a hexagonal unit cell, the structures of three other crystals
were determined by molecular replacement. In two cases, the rhombohedral and
cubic crystals, placement was facilitated by coincidence of threefold molecular
symmetry with crystallographic operators. In the orthorhombic crystal the
canavalin trimer was the asymmetric unit. The rhombohedral, orthorhombic and
cubic crystal structures were subsequently refined using a combination of
several approaches with resulting R factors of 0.194, 0.185 and 0.211 at
resolutions of 2.6, 2.6 and 2.3 A, respectively. Variation in the conformation
of the molecule from crystal to crystal was small with an r.m.s. deviation in
Calpha positions of 0.89 A. Packing is quite different among crystal forms but
lattice interactions appear to play little role in the conformation of the
molecule. Greatest variations in mean position are for those residues that also
exhibit the greatest thermal motion. Crystal contacts in all crystals are
mediated almost exclusively by hydrophilic side chains, and three to six
intermolecular salt bridges per protein subunit are present in each case.
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Figure 3.
Fig. 3. A schematic drawing of the canavalin subunit. The
polypeptide termini are labeled as N,, C~, ~ and C. The
triangle indicates the location of the riad axis around which
three monomers are arranged.
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Figure 7.
Fig. 7. A Ramachandran pot showing most ¢,~b anges to be
within allowed regions according t Morris et al. (1992).
Symbols: (A) R3, non-glycine residues; (A) R3, glycines; (11)
C222~, non-glycine residues; (r-q) C222~, glycines; (0) P2~3,
non-glycine resiues; (O) P2~3, glycines.
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The above figures are
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(1993,
49,
478-489)
copyright 1993.
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Secondary reference #1
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Title
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The three-Dimensional structure of canavalin from jack bean (canavalia ensiformis).
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Authors
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T.P.Ko,
J.D.Ng,
A.Mcpherson.
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Ref.
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Plant Physiol, 1993,
101,
729-744.
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PubMed id
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Note: In the PDB file this reference is
annotated as "TO BE PUBLISHED". The citation details given above have
been manually determined.
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Secondary reference #2
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Title
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The three-Dimensional structure of canavalin from jack bean (canavalia ensiformis).
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Authors
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T.P.Ko,
J.D.Ng,
A.Mcpherson.
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Ref.
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Plant Physiol, 1993,
101,
729-744.
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PubMed id
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