PDBsum entry 1c7g

Lyase

PDB id: 1c7g

Contents

Protein chains

456 a.a. *

Ligands

PLP ×4

Waters ×425

* Residue conservation analysis

PDB id:

1c7g

Name:

Lyase

Title:

Tyrosine phenol-lyase from erwinia herbicola

Structure:

Tyrosine phenol-lyase. Chain: a, b, c, d. Engineered: yes. Other_details: pyridoxal 5'-phosphate link to a 257, b 257, c 257 and d 257

Source:

Pantoea agglomerans pv. Gypsophilae. Organism_taxid: 48984. Strain: pv. Gypsophilae. Variant: aj2985. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_variant: tna deficient. Other_details: recombinant gene

Biol. unit:

Tetramer (from PQS)

Resolution:

2.10Å R-factor: 0.186 R-free: 0.242

Authors:

B.Mikami,Y.Yamamoto,T.Katayama,H.Suzuki

Key ref:

B.Mikami et al. The structure of tyrosine phenol-Lyase from erwinia herbicola. To be published, .

Date:

18-Feb-00 Release date: 09-Dec-03

Protein chains

P31011  (TPL_ENTAG) -  Tyrosine phenol-lyase from Enterobacter agglomerans

Seq: 456 a.a.

Struc: 456 a.a.

Enzyme reactions

• Enzyme class: E.C.4.1.99.2 - tyrosine phenol-lyase.
• Reaction: L-tyrosine + H2O = phenol + pyruvate + NH4⁺
• Cofactor: Pyridoxal 5'-phosphate

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = PLP) matches with 93.75% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site