UniProt functional annotation for P13342



	UniProt functional annotation for P13342

UniProt code: P13342.

Organism: Enterobacteria phage T4 (Bacteriophage T4).

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.

Function: DNA helicase loader protein that participates in viral DNA replication, recombination, and repair (PubMed:10871615). At the fork, required for loading of the replicative helicase onto DNA protected by the ssDNA-binding protein (PubMed:11459967, PubMed:7806533, PubMed:10871615, PubMed:22427673). Coordinates simultaneous synthesis of leading- and lagging-strands (PubMed:11459969). {ECO:0000269|PubMed:10871615, ECO:0000269|PubMed:11459967, ECO:0000269|PubMed:22427673, ECO:0000269|PubMed:7806533, ECO:0000303|PubMed:11459969}.

Subunit: Monomer (PubMed:19700405). Homohexamer; when associated with DNA (PubMed:19700405). Interacts (via C-terminus) with the DnaB-like replicative helicase (via C-terminus); this interaction brings about the rapid assembly of the helicase onto ssDNA (PubMed:11459967, PubMed:7806533, PubMed:10871615, PubMed:22427673). Interacts (via C- terminus) with the single-stranded DNA-binding protein; a ternary complex between the helicase assembly protein, the single-stranded DNA- binding protein and ssDNA is an obligatory intermediate in the helicase loading mechanism (PubMed:11459967, PubMed:24338568, PubMed:15507125, PubMed:7806533, PubMed:22427673). Interacts with the viral DNA polymerase (PubMed:15909989). Binds to single and double-stranded DNA (PubMed:10669611). Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single- stranded DNA binding protein (SSB), the primase, the DnaB-like replicative helicase and the helicase assembly factor (PubMed:16800624). {ECO:0000269|PubMed:10669611, ECO:0000269|PubMed:10871615, ECO:0000269|PubMed:11459967, ECO:0000269|PubMed:15507125, ECO:0000269|PubMed:15909989, ECO:0000269|PubMed:16800624, ECO:0000269|PubMed:19700405, ECO:0000269|PubMed:22427673, ECO:0000269|PubMed:24338568, ECO:0000269|PubMed:7806533}.

Similarity: Belongs to the Tequatrovirus DNA helicase assembly protein family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Enterobacteria phage T4 (Bacteriophage T4).
Taxonomy:		Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.



Function:		DNA helicase loader protein that participates in viral DNA replication, recombination, and repair (PubMed:10871615). At the fork, required for loading of the replicative helicase onto DNA protected by the ssDNA-binding protein (PubMed:11459967, PubMed:7806533, PubMed:10871615, PubMed:22427673). Coordinates simultaneous synthesis of leading- and lagging-strands (PubMed:11459969). {ECO:0000269\|PubMed:10871615, ECO:0000269\|PubMed:11459967, ECO:0000269\|PubMed:22427673, ECO:0000269\|PubMed:7806533, ECO:0000303\|PubMed:11459969}.


Subunit:		Monomer (PubMed:19700405). Homohexamer; when associated with DNA (PubMed:19700405). Interacts (via C-terminus) with the DnaB-like replicative helicase (via C-terminus); this interaction brings about the rapid assembly of the helicase onto ssDNA (PubMed:11459967, PubMed:7806533, PubMed:10871615, PubMed:22427673). Interacts (via C- terminus) with the single-stranded DNA-binding protein; a ternary complex between the helicase assembly protein, the single-stranded DNA- binding protein and ssDNA is an obligatory intermediate in the helicase loading mechanism (PubMed:11459967, PubMed:24338568, PubMed:15507125, PubMed:7806533, PubMed:22427673). Interacts with the viral DNA polymerase (PubMed:15909989). Binds to single and double-stranded DNA (PubMed:10669611). Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single- stranded DNA binding protein (SSB), the primase, the DnaB-like replicative helicase and the helicase assembly factor (PubMed:16800624). {ECO:0000269\|PubMed:10669611, ECO:0000269\|PubMed:10871615, ECO:0000269\|PubMed:11459967, ECO:0000269\|PubMed:15507125, ECO:0000269\|PubMed:15909989, ECO:0000269\|PubMed:16800624, ECO:0000269\|PubMed:19700405, ECO:0000269\|PubMed:22427673, ECO:0000269\|PubMed:24338568, ECO:0000269\|PubMed:7806533}.
Similarity:		Belongs to the Tequatrovirus DNA helicase assembly protein family. {ECO:0000305}.