PDBsum entry 1bxi

Immune system

PDB id: 1bxi

Contents

Protein chains

83 a.a. *

131 a.a. *

Ligands

PO4

Metals

_NI

Waters ×103

* Residue conservation analysis

PDB id:

1bxi

Name:

Immune system

Title:

Crystal structure of the escherichia coli colicin e9 dnase domain with its cognate immunity protein im9

Structure:

Protein (colicin e9 immunity protein). Chain: a. Engineered: yes. Protein (colicin e9). Chain: b. Fragment: dnase domain, residues 450-581. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 562. Gene: e9imm. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: cole9. Expression_system_variant: b834 (de3).

Biol. unit:

Dimer (from PQS)

Resolution:

2.05Å R-factor: 0.218 R-free: 0.315

Authors:

U.C.Kuhlmann,C.Kleanthous,R.James,G.R.Moore,A.M.Hemmings

Key ref:

U.C.Kuhlmann Crystal structure of the e.Coli colicin e9 dnase domain with its cognate immunity protein im9. Thesis, .

Date:

04-Oct-98 Release date: 04-Oct-99

Protein chain

P13479  (IMM9_ECOLX) -  Colicin-E9 immunity protein from Escherichia coli

Seq: 86 a.a.

Struc: 83 a.a.*

Protein chain

P09883  (CEA9_ECOLX) -  Colicin-E9 from Escherichia coli

Seq: 582 a.a.

Struc: 131 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain B: E.C.3.1.-.-