PDBsum entry 1bt4

Transferase

PDB id: 1bt4

Contents

Protein chain

361 a.a. *

Ligands

PLP

Waters ×98

* Residue conservation analysis

PDB id:

1bt4

Name:

Transferase

Title:

Phosphoserine aminotransferase from bacillus circulans subsp. Alkalophilus

Structure:

Phosphoserine aminotransferase. Chain: a. Fragment: one complete subunit. Synonym: phosphohydroxythreonine aminotransferase, psat. Mutation: yes. Other_details: the cofactor pyridoxal-5'-phosphate is covalently linked to the side chain of lys 197

Source:

Bacillus circulans. Organism_taxid: 1397. Strain: subsp. Alkalophilus. Variant: alkalophilus. Atcc: atcc 21783. Collection: atcc 21783

Biol. unit:

Dimer (from PDB file)

Resolution:

2.30Å R-factor: 0.184 R-free: 0.246

Authors:

G.Hester,T.N.Luong,M.Moser,J.N.Jansonius

Key ref:

G.Hester et al. The crystal structure of phosphoserine aminotransferase from bacillus circulans subsp. Alkalophilus. To be published, .

Date:

02-Sep-98 Release date: 09-Sep-98

Protein chain

Q59196  (SERC_NIACI) -  Phosphoserine aminotransferase from Niallia circulans

Seq: 362 a.a.

Struc: 361 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.6.1.52 - phosphoserine transaminase.
• Reaction:
  1. O-phospho-L-serine + 2-oxoglutarate = 3-phosphooxypyruvate + L-glutamate
  2. 4-(phosphooxy)-L-threonine + 2-oxoglutarate = (R)-3-hydroxy-2-oxo-4- phosphooxybutanoate + L-glutamate
• Cofactor: Pyridoxal 5'-phosphate

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = PLP) matches with 93.75% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site