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UniProt functional annotation for P32055 | ||
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| UniProt code: P32055. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Catalyzes the two-step NADP-dependent conversion of GDP-4- dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction. {ECO:0000255|HAMAP-Rule:MF_00956, ECO:0000269|PubMed:10480878, ECO:0000269|PubMed:11021971, ECO:0000269|PubMed:9473059}. |
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| Catalytic activity: | |
Reaction=GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-alpha-D-rhamnose + H(+) + NADPH; Xref=Rhea:RHEA:18885, ChEBI:CHEBI:15378, ChEBI:CHEBI:57273, ChEBI:CHEBI:57783, ChEBI:CHEBI:57964, ChEBI:CHEBI:58349; EC=1.1.1.271; Evidence={ECO:0000255|HAMAP- Rule:MF_00956, ECO:0000269|PubMed:10480878, ECO:0000269|PubMed:11021971, ECO:0000269|PubMed:9473059}; |
| Activity regulation: | |
Subject to product inhibition by NADP and GDP- fucose. {ECO:0000269|PubMed:10480878}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=9 uM for NADPH {ECO:0000269|PubMed:10480878, ECO:0000269|PubMed:11021971}; KM=109 uM for NADH {ECO:0000269|PubMed:10480878, ECO:0000269|PubMed:11021971}; KM=29 uM for GDP-4-keto-6-deoxymannose {ECO:0000269|PubMed:10480878, ECO:0000269|PubMed:11021971}; Vmax=363.5 umol/min/mg enzyme {ECO:0000269|PubMed:10480878, ECO:0000269|PubMed:11021971}; pH dependence: Optimum pH is 6-6.5. {ECO:0000269|PubMed:10480878, ECO:0000269|PubMed:11021971}; |
| Pathway: | |
Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 2/2. {ECO:0000255|HAMAP-Rule:MF_00956, ECO:0000269|PubMed:9473059}. |
| Pathway: | |
Exopolysaccharide biosynthesis; colanic acid biosynthesis. {ECO:0000269|PubMed:9473059}. |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:11021971, ECO:0000269|PubMed:9817848, ECO:0000269|PubMed:9862812}. |
| Subcellular location: | |
Cytoplasm. |
| Similarity: | |
Belongs to the NAD(P)-dependent epimerase/dehydratase family. Fucose synthase subfamily. {ECO:0000255|HAMAP-Rule:MF_00956}. |
Annotations taken from UniProtKB at the EBI.