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PDBsum entry 1bje
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Oxygen transport
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PDB id
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1bje
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References listed in PDB file
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Key reference
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Title
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Structural and spectroscopic studies of azide complexes of horse heart myoglobin and the his-64--≫thr variant.
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Authors
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R.Maurus,
R.Bogumil,
N.T.Nguyen,
A.G.Mauk,
G.Brayer.
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Ref.
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Biochem J, 1998,
332,
67-74.
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PubMed id
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Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
perfect match.
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Abstract
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The high-resolution X-ray crystallographic structures of horse heart
azidometmyoglobin complexes of the wild-type protein and the His-64-->Thr
variant have been determined to 2.0 and 1.8 A respectively. Azide binds to
wild-type metmyoglobin in a bent configuration with an Fe-N-1-N-3 angle of 119
degrees and is oriented into the distal crevice in the direction of Ile-107. The
proximity of the His-64 NE2 atom to the N-1 atom of the bound azide indicates
stabilization of the ligand by the His-64 side chain through hydrogen bonding.
In addition, structural characterization of wild-type horse heart
azidometmyoglobin establishes that the only structural change induced by ligand
binding is a small movement of the Leu-29 side chain away from the azide ligand.
EPR and Fourier transform infrared spectroscopy were used to characterize the
myoglobin azide complexes further. EPR spectroscopy revealed that, in contrast
with wild-type azidometmyoglobin, two slightly different low-spin species are
formed by azide bound to the His-64-->Thr variant both in solution and in a
polycrystalline sample. One of these low-spin species has a greater relative
intensity, with g values very similar to those of the azide complex of the
wild-type protein. These EPR results together with structural information on
this variant indicate the presence of two distinct conformations of bound azide,
with one form predominating. The major conformation is comparable to that formed
by wild-type myoglobin in which azide is oriented into the distal crevice. In
the minor conformation the azide is oriented towards the exterior of the protein.
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Secondary reference #1
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Title
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A myoglobin variant with a polar substitution in a conserved hydrophobic cluster in the heme binding pocket.
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Authors
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R.Maurus,
C.M.Overall,
R.Bogumil,
Y.Luo,
A.G.Mauk,
M.Smith,
G.D.Brayer.
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Ref.
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Biochim Biophys Acta, 1997,
1341,
1.
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PubMed id
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Secondary reference #2
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Title
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Structural characterization of heme ligation in the his64--≫tyr variant of myoglobin.
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Authors
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R.Maurus,
R.Bogumil,
Y.Luo,
H.L.Tang,
M.Smith,
A.G.Mauk,
G.D.Brayer.
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Ref.
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J Biol Chem, 1994,
269,
12606-12610.
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PubMed id
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Secondary reference #3
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Title
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Ftir analysis of the interaction of azide with horse heart myoglobin variants.
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Authors
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R.Bogumil,
C.L.Hunter,
R.Maurus,
H.L.Tang,
H.Lee,
E.Lloyd,
G.D.Brayer,
M.Smith,
A.G.Mauk.
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Ref.
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Biochemistry, 1994,
33,
7600-7608.
[DOI no: ]
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PubMed id
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Secondary reference #4
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Title
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Three-Dimensional structure of cyanomet-Sulfmyoglobin c.
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Authors
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S.V.Evans,
B.P.Sishta,
A.G.Mauk,
G.D.Brayer.
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Ref.
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Proc Natl Acad Sci U S A, 1994,
91,
4723-4726.
[DOI no: ]
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PubMed id
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Secondary reference #5
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Title
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Expression in escherichia coli of a synthetic gene coding for horse heart myoglobin.
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Authors
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J.G.Guillemette,
Y.Matsushima-Hibiya,
T.Atkinson,
M.Smith.
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Ref.
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Protein Eng, 1991,
4,
585-592.
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PubMed id
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Secondary reference #6
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Title
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High-Resolution study of the three-Dimensional structure of horse heart metmyoglobin.
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Authors
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S.V.Evans,
G.D.Brayer.
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Ref.
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J Mol Biol, 1990,
213,
885-897.
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PubMed id
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Secondary reference #7
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Title
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Horse heart metmyoglobin. A 2.8-A resolution three-Dimensional structure determination.
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Authors
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S.V.Evans,
G.D.Brayer.
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Ref.
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J Biol Chem, 1988,
263,
4263-4268.
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PubMed id
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Secondary reference #8
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Title
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Crystallization and preliminary diffraction data for horse heart metmyoglobin.
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Authors
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C.Sherwood,
A.G.Mauk,
G.D.Brayer.
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Ref.
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J Mol Biol, 1987,
193,
227.
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PubMed id
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